RNF111/Arkadia is regulated by DNA methylation and affects TGF-β/Smad signaling associated invasion in NSCLC cells

Highlights • Methylation level of -459CpG is related to RNF111 mRNA expression in NSCLC cells. • Sp1 binds RNF111 promoter containing -459CpG and upregulates RNF111 expression. • -459CpG methylation inhibits the recruitment of Sp1 to RNF111 promoter. • RNF111 knockdown represses TGF-β/Smad signaling...

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Bibliographic Details
Published in:Lung cancer (Amsterdam, Netherlands) Netherlands), 2015-10, Vol.90 (1), p.32-40
Main Authors: Chen, Hongbing, Yang, Tianjie, Lei, Zhe, Wang, Longqiang, Yang, Haiping, Tong, Xin, Yang, Wen-Tao, Zhao, Jun, Gu, Yunbin, Chen, Yongbing, Zhang, Hong-Tao
Format: Article
Language:English
Subjects:
459CpG methylation
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Non-Small-Cell Lung - metabolism
Carcinoma, Non-Small-Cell Lung - pathology
Cell Line, Tumor
DNA Methylation
Epithelial-Mesenchymal Transition
Hematology, Oncology and Palliative Medicine
Humans
Invasion
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Mutation
Neoplasm Invasiveness
NSCLC
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Phosphorylation
Promoter Regions, Genetic
Pulmonary/Respiratory
RNA Interference
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNF111
Smad3 Protein - genetics
Smad3 Protein - metabolism
TGF-β/Smad signaling
Transforming Growth Factor beta - genetics
Transforming Growth Factor beta - metabolism
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
Up-Regulation
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Summary:Highlights • Methylation level of -459CpG is related to RNF111 mRNA expression in NSCLC cells. • Sp1 binds RNF111 promoter containing -459CpG and upregulates RNF111 expression. • -459CpG methylation inhibits the recruitment of Sp1 to RNF111 promoter. • RNF111 knockdown represses TGF-β/Smad signaling activity and NSCLC cell invasion.
ISSN:0169-5002
1872-8332
DOI:10.1016/j.lungcan.2015.07.010