Antinociceptive and anti-inflammatory effects of myricetin 3-O-β-galactoside isolated from Davilla elliptica: involvement of the nitrergic system

• Published in: Journal of natural medicines 2015-10, Vol.69 (4), p.487-493
• Main Authors: de Oliveira Azevedo, Adolfo, Campos, Jussara Júlia, de Souza, Giovane Galdino, de Carvalho Veloso, Clarice, Duarte, Igor Dimitri Gama, Braga, Fernão Castro, de Castro Perez, Andrea
• Format: Article
• Language: English
• Subjects: Analgesics - metabolism; Animals; Anti-Inflammatory Agents - metabolism; Biomedical and Life Sciences; Biomedicine; Complementary & Alternative Medicine; Edema - drug therapy; Galactosides - chemistry; Male; Medicinal Chemistry; Mice; Mice, Inbred C57BL; Mice, Knockout; Nitric Oxide - chemistry; Original Paper; Pharmacology/Toxicology; Pharmacy; Plant Sciences; Plants, Medicinal - chemistry
• ISSN: 1340-3443; 1861-0293
• DOI: 10.1007/s11418-015-0913-9

We aimed to study the antinociceptive effects of myricetin 3- O -β-galactoside (Mi), a substance isolated from the hydroalcoholic extract of Davilla elliptica . This study examined male Swiss mice, inducible nitric oxide synthase C57B16/J knockout mice (iNOS −/− ), and their corresponding wild type (WT). Formalin and tail-flick tests were used to evaluate the nociceptive threshold, and the carrageenan-induced paw edema test was used as a model for inflammation. The following drugs were administered to investigate the involvement of the nitrergic and opioidergic systems: l -NAME, a nonspecific nitric oxide synthase (NOS) inhibitor; l -arginine ( l -Arg), a precursor for the synthesis of nitric oxide (NO); d -arginine ( d -Arg), an inactive isomer for the synthesis of NO; aminoguanidine (Am), an inducible nitric oxide synthase (iNOS) inhibitor; and naloxone, a nonselective antagonist of opioid receptors. The results showed that oral pretreatment with Mi caused a dose-dependent inhibition of the inflammatory phase of the formalin test and did not alter motor performance. Intraperitoneal injection of l -NAME caused a reduction in the licking time during the second phase of the formalin test. The administration of l -Arg (but not d -Arg) reversed the antinociceptive effect of l -NAME. Furthermore, pre-administration of aminoguanidine potentiated the antinociceptive effect. Mi did not cause an antinociceptive effect in iNOS knockouts and led to a reduction in the nitrite concentration in the paws of mice. Carrageenan-induced paw edema was reduced in Swiss mice and WT mice when compared to iNOS −/− mice. Pre-administration of naloxone (NLX) did not reverse the antinociceptive effect of Mi, excluding the opioidergic system as a mediator of the antinociceptive effect. Thus, the results suggest that the antinociceptive and anti-inflammatory effects of myricetin 3- O -β-galactoside are related to peripheral inhibition of nitric oxide synthesis, mainly iNOS.