Development of primary shrimp hemocyte cultures of Penaeus chinensis to study white spot syndrome virus (WSSV) infection

Hemocytes were withdrawn from hearts of Penaeus chinensis, cultured with seven kinds of culture media, the hemocytes remained viable for about 20 days using Leibovitz's 2 × L-15 medium consisting of 20% fetal bovine serum, 20% GIT, and 2% glucose, adjusting osmolarity with sodium chloride, pH 7...

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Bibliographic Details
Published in:Aquaculture 2006-03, Vol.253 (1), p.114-119
Main Authors: Jiang, You-Sheng, Zhan, Wen-Bin, Wang, Shi-Biao, Xing, Jing
Format: Article
Language:English
Subjects:
Animal aquaculture
Animal productions
Aquaculture
Biological and medical sciences
cell culture
Crustacea
culture media
Fenneropenaeus chinensis
Fundamental and applied biological sciences. Psychology
General aspects
Heart
Hemocytes
Immunocytochemical tests
immunocytochemistry
Immunology
Infection
Infections
Invertebrates
Marine
Shellfish
shrimp
shrimp culture
Viruses
white spot syndrome
White spot syndrome virus
WSSV
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Summary:Hemocytes were withdrawn from hearts of Penaeus chinensis, cultured with seven kinds of culture media, the hemocytes remained viable for about 20 days using Leibovitz's 2 × L-15 medium consisting of 20% fetal bovine serum, 20% GIT, and 2% glucose, adjusting osmolarity with sodium chloride, pH 7.2–7.4, and incubated at a temperature of 25 °C. Different concentrations of virus inoculum were inoculated into the primary cultured hemocytes, and diverse cytopathic effects were observed with an inverted phase-contrast microscope. WSSV infection was confirmed by immunocytochemical tests with monoclonal antibodies against the virus. These revealed that WSSV could infect hemocytes of P. chinensis and propagate in the nuclei of infected cells.
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2005.07.045