Cloning and nucleotide sequence of the endoinulinase-encoding gene, inu2, from Aspergillus ficuum

A 2.3 kb DNA fragment that contains a gene encoding endoinulinase, inu2, from Aspergillus ficuum ATCC 16882 was isolated and analyzed. It includes an open reading frame of 1,551 bp, coding for a polypeptide with calculated molecular weight of 55,790 Da, including a putative signal peptide of 22 amin...

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Bibliographic Details
Published in:Biotechnology letters 1998-08, Vol.20 (8), p.809-812
Main Authors: UHM, T. B, CHAE, K.-S, LEE, D. W, KIM, H.-S, CASSART, J.-P, VANDENHAUTE, J
Format: Article
Language:English
Subjects:
Amino acids
Aspergillus ficuum
Bacteria
Bacteriology
Biological and medical sciences
Biotechnology
Cloning
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Penicillium purpurogenum
Synthetic digonucleotides and genes. Sequencing
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Summary:A 2.3 kb DNA fragment that contains a gene encoding endoinulinase, inu2, from Aspergillus ficuum ATCC 16882 was isolated and analyzed. It includes an open reading frame of 1,551 bp, coding for a polypeptide with calculated molecular weight of 55,790 Da, including a putative signal peptide of 22 amino acids. Alignment of amino acid sequences revealed 73.3% identity and 93.9% similarity between A. ficuum and Penicillium purpurogenum endoinulinase.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/b:bile.0000015928.45007.30