Optimization of carotenoid production by Rhodotorula glutinis using statistical experimental design

A two-step optimization strategy of statistical experimental design was employed to enhance carotenoid production from sugar cane molasses (SCM) in the yeast Rhodotorula glutinis. In the first step, a fractional factorial design was used to evaluate the impact of five fermentation factors (pH and co...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2005-06, Vol.21 (4), p.429-434
Main Authors: PARK, P. K, CHO, D. H, KIM, E. Y, CHU, K. H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Carotenoids
Design of experiments
Experimental design
Fermentation
Fundamental and applied biological sciences. Psychology
Optimization
Rhodotorula glutinis
Sodium chloride
Sugarcane
Urea
Yeasts
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Summary:A two-step optimization strategy of statistical experimental design was employed to enhance carotenoid production from sugar cane molasses (SCM) in the yeast Rhodotorula glutinis. In the first step, a fractional factorial design was used to evaluate the impact of five fermentation factors (pH and concentrations of SCM, urea, KH^sub 2^PO^sub 4^, and NaCl). The results revealed that three factors (concentrations of SCM, urea, and KH^sub 2^PO^sub 4^) had a significant influence on biomass and carotenoid production. A face-centered central composite design was then used in the second step to optimize the three significant factors to further enhance the biomass yield and carotenoid production. Through this two-step optimization strategy, the carotenoid concentration could be increased from an average of 1.39 mg/l to an average of 3.46 mg/l, representing a 2.5-fold carotenoid production enhancement.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-004-1891-3