A novel Candida glycerinogenes mutant with high glycerol productivity in high phosphate concentration medium

A novel Candida glycerinogenes mutant, which possesses high glycerol productivity in a high phosphate concentration medium, was obtained by mutagenesis of an industrial glycerol producer. The mutant accumulated a total biomass of 11.5 g l^sup -1^, which is less than the 15 g l^sup -1^of the wild-typ...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2005-06, Vol.21 (4), p.453-456
Main Authors: BIN ZHUGE, GUO, Xue-Na, MAWADZA, Crispen, FANG, Hui-Ying, TANG, Xue-Ming, ZHANG, Xi-Hong, JIANG ZHUGE
Format: Article
Language:English
Subjects:
Bacteria
Bacteriology
Biological and medical sciences
Biotechnology
Candida glycerinogenes
Fermentation
Fundamental and applied biological sciences. Psychology
Glycerol
Mutants
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Summary:A novel Candida glycerinogenes mutant, which possesses high glycerol productivity in a high phosphate concentration medium, was obtained by mutagenesis of an industrial glycerol producer. The mutant accumulated a total biomass of 11.5 g l^sup -1^, which is less than the 15 g l^sup -1^of the wild-type strain, but it consumed glucose faster than the wild-type strain did. The mutant reached its maximal glycerol concentration of 129 g l^sup -1^ in 84 h compared to 96 h for the wild-type strain. High cytoplasmic glycerol-3-phosphate dehydrogenase activity of the mutant in the early glycerol formation phase, leading to a rapid glycerol synthesis and accumulation, may be the main reason for the short fermentation process.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-004-1879-z