Genetic diversity of Pakistan wheat germplasm as revealed by RAPD markers

Wheat breeding in Pakistan started in 1930s before partition in the United India and so far has released more than 68 cultivars, but no systematic analyses of the genetic diversity of Pakistan wheat have been made. Twenty Pakistan wheat cultivars released from 1933 to 2002 were examined for genetic...

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Bibliographic Details
Published in:Genetic resources and crop evolution 2005-05, Vol.52 (3), p.239-244
Main Authors: Khan, I.A, Awan, F.S, Ahmad, A, Fu, Y.B, Iqbal, A
Format: Article
Language:English
Subjects:
breeding lines
Cultivars
Deoxyribonucleic acid
DNA
Genetic analysis
Genetic diversity
genetic markers
genetic polymorphism
genetic variation
Germplasm
Markers
Plant breeding
Polymerase chain reaction
Primers
Random amplified polymorphic DNA
random amplified polymorphic DNA technique
Statistical analysis
Triticum aestivum
Wheat
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Summary:Wheat breeding in Pakistan started in 1930s before partition in the United India and so far has released more than 68 cultivars, but no systematic analyses of the genetic diversity of Pakistan wheat have been made. Twenty Pakistan wheat cultivars released from 1933 to 2002 were examined for genetic diversity and relationships using random amplified polymorphic DNA (RAPD) markers. Forty-two RAPD primers were applied and 184 polymorphic bands were generated for each cultivar. Most of the cultivars were genetically interrelated, although six of them displayed some genetic distinctness. The RAPD variation observed among these cultivars was low. Only 40.7% of the total scorable bands were polymorphic, and 26.1% of the polymorphic bands were observed most frequently (f = 0.95) among the 20 cultivars. The proportions of polymorphic bands for each cultivar ranged from 0.67 in 'Yecora' to 0.84 in 'C-250' with an average of 0.76. About 1.4% of the RAPD variation might have been fixed over the 69 years of wheat breeding, but such fixation was not statistically significant. These results are significant for future improvement and conservation of Pakistan wheat.
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-004-5730-z