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Partial characterization of enterocin MR99 from a corn silage isolate of Enterococcus faecalis

Aims:  To assess the inhibitory activity on Gram‐positive and Gram‐negative bacteria of several species of enterococci recovered from a natural corn silage. Methods and Results:  The inhibitory activity of strains of Enterococcus faecalis (58), Enterococcus faecium (35), Enterococcus gallinarum (3)...

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Published in:Journal of applied microbiology 2006-01, Vol.100 (1), p.123-134
Main Authors: Sparo, M.D., Castro, M.S., Andino, P.J., Lavigne, M.V., Ceriani, C., Gutiérrez, G.L., Fernández, M.M., De Marzi, M.C., Malchiodi, E.L., Manghi, M.A.
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container_title Journal of applied microbiology
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creator Sparo, M.D.
Castro, M.S.
Andino, P.J.
Lavigne, M.V.
Ceriani, C.
Gutiérrez, G.L.
Fernández, M.M.
De Marzi, M.C.
Malchiodi, E.L.
Manghi, M.A.
description Aims:  To assess the inhibitory activity on Gram‐positive and Gram‐negative bacteria of several species of enterococci recovered from a natural corn silage. Methods and Results:  The inhibitory activity of strains of Enterococcus faecalis (58), Enterococcus faecium (35), Enterococcus gallinarum (3) and Enterococcus casseliflavus (4) were studied employing indicator strains from various sources (clinical, food and ATCC). Enterococcus faecalis MR99, the only strain with inhibitory activity, inhibited other enterococci, Listeria spp., Staphylococcus aureus, Clostridium spp., Bacillus spp., Escherichia coli, Shigella sonnei and Shigella flexneri. The bacterium contained only one conjugative pheromone‐responsive plasmid. The partially chromatography‐purified MR99 enterocin (PPE) had a molecular weight of approx. 5000 Da and a pI of 6·2, was sensitive to proteolytic enzymes and could be extracted in benzene and butanol. It appeared stable to adjustment of pH 4·0, 5·0, 6·0, 7·0 and 8·0 and was resistant to heat. Inactivation was at 15 min at 121°C. Enterocin MR99 was bactericidal on strains of Listeria monocytogenes, Staph. aureus, and bovine mastitis agents, it was bacteriostatic on E. coli. Although enterocins MR99 and AS48 have inhibitory activity on Gram‐negative bacilli, PCR studies demonstrated a lack of relationship between them. Conclusions:  The active component had a protein nature, was resistant to heat and presented a wide inhibitory spectrum. Significance and Impact of the Study:  The biological properties of Ent. faecalis MR99 suggest that this strain merits further investigations so it can be applied in human and veterinary health programmes.
doi_str_mv 10.1111/j.1365-2672.2005.02752.x
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Methods and Results:  The inhibitory activity of strains of Enterococcus faecalis (58), Enterococcus faecium (35), Enterococcus gallinarum (3) and Enterococcus casseliflavus (4) were studied employing indicator strains from various sources (clinical, food and ATCC). Enterococcus faecalis MR99, the only strain with inhibitory activity, inhibited other enterococci, Listeria spp., Staphylococcus aureus, Clostridium spp., Bacillus spp., Escherichia coli, Shigella sonnei and Shigella flexneri. The bacterium contained only one conjugative pheromone‐responsive plasmid. The partially chromatography‐purified MR99 enterocin (PPE) had a molecular weight of approx. 5000 Da and a pI of 6·2, was sensitive to proteolytic enzymes and could be extracted in benzene and butanol. It appeared stable to adjustment of pH 4·0, 5·0, 6·0, 7·0 and 8·0 and was resistant to heat. Inactivation was at 15 min at 121°C. Enterocin MR99 was bactericidal on strains of Listeria monocytogenes, Staph. aureus, and bovine mastitis agents, it was bacteriostatic on E. coli. Although enterocins MR99 and AS48 have inhibitory activity on Gram‐negative bacilli, PCR studies demonstrated a lack of relationship between them. Conclusions:  The active component had a protein nature, was resistant to heat and presented a wide inhibitory spectrum. Significance and Impact of the Study:  The biological properties of Ent. faecalis MR99 suggest that this strain merits further investigations so it can be applied in human and veterinary health programmes.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2005.02752.x</identifier><identifier>PMID: 16405692</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Bacillus ; Bacillus - drug effects ; bacteriocin ; Bacteriocins - pharmacology ; Biological and medical sciences ; Bridged-Ring Compounds - analysis ; Bridged-Ring Compounds - pharmacology ; Clostridium ; Clostridium - drug effects ; corn silage ; Culture Media ; Electrophoresis, Polyacrylamide Gel - methods ; Enterocins screening ; Enterococcus - physiology ; Enterococcus casseliflavus ; Enterococcus faecalis ; Enterococcus faecalis - physiology ; Enterococcus faecalis MR99 ; Enterococcus faecium ; Enterococcus faecium - physiology ; Enterococcus gallinarum ; Escherichia coli ; Escherichia coli - drug effects ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Isoelectric Focusing - methods ; Listeria - drug effects ; Listeria monocytogenes ; Listeria monocytogenes - drug effects ; Listeria monocytogenes - ultrastructure ; Microbiology ; Microscopy, Electron - methods ; Plasmids - isolation &amp; purification ; Polymerase Chain Reaction - methods ; Shigella - drug effects ; Shigella flexneri ; Shigella sonnei ; Silage - microbiology ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - ultrastructure ; Zea mays - microbiology</subject><ispartof>Journal of applied microbiology, 2006-01, Vol.100 (1), p.123-134</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Jan 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5212-190801a704a7fccc96668cae820a448cc88ddbc80948341d3f81d4cdf78a42ab3</citedby><cites>FETCH-LOGICAL-c5212-190801a704a7fccc96668cae820a448cc88ddbc80948341d3f81d4cdf78a42ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=17476865$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16405692$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sparo, M.D.</creatorcontrib><creatorcontrib>Castro, M.S.</creatorcontrib><creatorcontrib>Andino, P.J.</creatorcontrib><creatorcontrib>Lavigne, M.V.</creatorcontrib><creatorcontrib>Ceriani, C.</creatorcontrib><creatorcontrib>Gutiérrez, G.L.</creatorcontrib><creatorcontrib>Fernández, M.M.</creatorcontrib><creatorcontrib>De Marzi, M.C.</creatorcontrib><creatorcontrib>Malchiodi, E.L.</creatorcontrib><creatorcontrib>Manghi, M.A.</creatorcontrib><title>Partial characterization of enterocin MR99 from a corn silage isolate of Enterococcus faecalis</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims:  To assess the inhibitory activity on Gram‐positive and Gram‐negative bacteria of several species of enterococci recovered from a natural corn silage. Methods and Results:  The inhibitory activity of strains of Enterococcus faecalis (58), Enterococcus faecium (35), Enterococcus gallinarum (3) and Enterococcus casseliflavus (4) were studied employing indicator strains from various sources (clinical, food and ATCC). Enterococcus faecalis MR99, the only strain with inhibitory activity, inhibited other enterococci, Listeria spp., Staphylococcus aureus, Clostridium spp., Bacillus spp., Escherichia coli, Shigella sonnei and Shigella flexneri. The bacterium contained only one conjugative pheromone‐responsive plasmid. The partially chromatography‐purified MR99 enterocin (PPE) had a molecular weight of approx. 5000 Da and a pI of 6·2, was sensitive to proteolytic enzymes and could be extracted in benzene and butanol. It appeared stable to adjustment of pH 4·0, 5·0, 6·0, 7·0 and 8·0 and was resistant to heat. Inactivation was at 15 min at 121°C. 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Psychology</topic><topic>Isoelectric Focusing - methods</topic><topic>Listeria - drug effects</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - drug effects</topic><topic>Listeria monocytogenes - ultrastructure</topic><topic>Microbiology</topic><topic>Microscopy, Electron - methods</topic><topic>Plasmids - isolation &amp; purification</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Shigella - drug effects</topic><topic>Shigella flexneri</topic><topic>Shigella sonnei</topic><topic>Silage - microbiology</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - ultrastructure</topic><topic>Zea mays - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sparo, M.D.</creatorcontrib><creatorcontrib>Castro, M.S.</creatorcontrib><creatorcontrib>Andino, P.J.</creatorcontrib><creatorcontrib>Lavigne, M.V.</creatorcontrib><creatorcontrib>Ceriani, C.</creatorcontrib><creatorcontrib>Gutiérrez, G.L.</creatorcontrib><creatorcontrib>Fernández, M.M.</creatorcontrib><creatorcontrib>De Marzi, M.C.</creatorcontrib><creatorcontrib>Malchiodi, E.L.</creatorcontrib><creatorcontrib>Manghi, M.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sparo, M.D.</au><au>Castro, M.S.</au><au>Andino, P.J.</au><au>Lavigne, M.V.</au><au>Ceriani, C.</au><au>Gutiérrez, G.L.</au><au>Fernández, M.M.</au><au>De Marzi, M.C.</au><au>Malchiodi, E.L.</au><au>Manghi, M.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Partial characterization of enterocin MR99 from a corn silage isolate of Enterococcus faecalis</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2006-01</date><risdate>2006</risdate><volume>100</volume><issue>1</issue><spage>123</spage><epage>134</epage><pages>123-134</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims:  To assess the inhibitory activity on Gram‐positive and Gram‐negative bacteria of several species of enterococci recovered from a natural corn silage. Methods and Results:  The inhibitory activity of strains of Enterococcus faecalis (58), Enterococcus faecium (35), Enterococcus gallinarum (3) and Enterococcus casseliflavus (4) were studied employing indicator strains from various sources (clinical, food and ATCC). Enterococcus faecalis MR99, the only strain with inhibitory activity, inhibited other enterococci, Listeria spp., Staphylococcus aureus, Clostridium spp., Bacillus spp., Escherichia coli, Shigella sonnei and Shigella flexneri. The bacterium contained only one conjugative pheromone‐responsive plasmid. The partially chromatography‐purified MR99 enterocin (PPE) had a molecular weight of approx. 5000 Da and a pI of 6·2, was sensitive to proteolytic enzymes and could be extracted in benzene and butanol. It appeared stable to adjustment of pH 4·0, 5·0, 6·0, 7·0 and 8·0 and was resistant to heat. Inactivation was at 15 min at 121°C. Enterocin MR99 was bactericidal on strains of Listeria monocytogenes, Staph. aureus, and bovine mastitis agents, it was bacteriostatic on E. coli. Although enterocins MR99 and AS48 have inhibitory activity on Gram‐negative bacilli, PCR studies demonstrated a lack of relationship between them. Conclusions:  The active component had a protein nature, was resistant to heat and presented a wide inhibitory spectrum. Significance and Impact of the Study:  The biological properties of Ent. faecalis MR99 suggest that this strain merits further investigations so it can be applied in human and veterinary health programmes.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>16405692</pmid><doi>10.1111/j.1365-2672.2005.02752.x</doi><tpages>12</tpages></addata></record>
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source Alma/SFX Local Collection
subjects Bacillus
Bacillus - drug effects
bacteriocin
Bacteriocins - pharmacology
Biological and medical sciences
Bridged-Ring Compounds - analysis
Bridged-Ring Compounds - pharmacology
Clostridium
Clostridium - drug effects
corn silage
Culture Media
Electrophoresis, Polyacrylamide Gel - methods
Enterocins screening
Enterococcus - physiology
Enterococcus casseliflavus
Enterococcus faecalis
Enterococcus faecalis - physiology
Enterococcus faecalis MR99
Enterococcus faecium
Enterococcus faecium - physiology
Enterococcus gallinarum
Escherichia coli
Escherichia coli - drug effects
Food Microbiology
Fundamental and applied biological sciences. Psychology
Isoelectric Focusing - methods
Listeria - drug effects
Listeria monocytogenes
Listeria monocytogenes - drug effects
Listeria monocytogenes - ultrastructure
Microbiology
Microscopy, Electron - methods
Plasmids - isolation & purification
Polymerase Chain Reaction - methods
Shigella - drug effects
Shigella flexneri
Shigella sonnei
Silage - microbiology
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - ultrastructure
Zea mays - microbiology
title Partial characterization of enterocin MR99 from a corn silage isolate of Enterococcus faecalis
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