Polymorphisms and allele frequencies of glutathione S-transferases A1 and P1 genes in the Polish population

• Published in: Genetics and molecular research 2015-03, Vol.14 (1), p.2850-2859
• Main Authors: Skrzypczak-Zielinska, M, Zakerska-Banaszak, O, Tamowicz, B, Sobieraj, I, Drweska-Matelska, N, Szalata, M, Slomski, R, Mikstacki, A
• Format: Article
• Language: English
• Subjects: Alleles; European Continental Ancestry Group - genetics; Gene Frequency; Genotype; Glutathione S-Transferase pi - genetics; Glutathione Transferase - genetics; Haplotypes; Humans; Linkage Disequilibrium; Poland; Polymorphism, Single Nucleotide; Promoter Regions, Genetic - genetics; Sequence Analysis, DNA
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2015.March.31.15

Glutathione S-transferases (GST) A1 and P1 are crucial enzymes involved in the biotransformation of drugs, carcinogens, and toxins, and their activity may influence drug response, susceptibility to diseases, and carcinogenesis. The genes encoding these enzymes, GSTA1 and GSTP1, have been examined in many studies because of their genetic variability, which may affect enzymatic activity. The goal of this study was to determine the distribution of the alleles GSTA1*A/*B and GSTP1*A, *B, and *C in the Polish population. A total of 160 subjects from the Polish population were genotyped for 2 polymorphisms (I105V and A114V) in the GSTP1 gene using pyrosequencing. The promoter region of the GSTA1 gene was screened using sequencing. The detected variants were subjected to haplotype analysis. We found that the distribution of the alleles GSTA1*A/*B and GSTP1*A, *B, and *C in the Polish population correspond to the results of studies in Caucasians. Furthermore, we identified additional single nucleotide polymorphisms, excluding 3 well-known changes (G-52A, C-69T, T-567G), which are linked to alleles GSTA1*A/*B, that affect enzyme activity. A total of 4 haplotypes were identified in 160 Polish individuals.