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Effectiveness of microsatellite and single nucleotide polymorphism markers for parentage analysis in European domestic pigs
Parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. Parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the lo...
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Published in: | Genetics and molecular research 2015-02, Vol.14 (1), p.1362-1370 |
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creator | Yu, G C Tang, Q Z Long, K R Che, T D Li, M Z Shuai, S R |
description | Parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. Parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the low mutation rate and genotyping error rate of single nucleotide polymorphisms (SNPs) make them another important marker for pedigree tracing. Here, we compared the effectiveness of microsatellites and SNP markers in European pigs. We also measured and presented the minimum and optimal criteria for SNP markers to be used in paternity and identity analysis. Our findings may contribute to the development of techniques for future molecular evolution and conservation studies, as well as breeding programs. |
doi_str_mv | 10.4238/2015.February.13.15 |
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subjects | Animals Breeding Europe Female Gene Frequency Genotype Male Microsatellite Repeats - genetics Models, Genetic Pedigree Polymorphism, Single Nucleotide Probability Sequence Analysis, DNA Sus scrofa - genetics |
title | Effectiveness of microsatellite and single nucleotide polymorphism markers for parentage analysis in European domestic pigs |
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