Neuroprotective activities of fermented Ganoderma lucidum extracts by lactic acid bacteria against H sub(2) O sub(2) -stimulated oxidative stress in PC12 cells

The purpose of this study was to elucidate the neuroprotective activities of Ganoderma lucidum water extract (GW) and fermented G. lucidum water extract (FGW). GW was fermented by the lactic acid bacterium Bifidobacterium bifidum (FGWB), followed by Lactobacillus sakei subsp. sakei LI033 (FGWBL). GW...

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Bibliographic Details
Published in:Food science and biotechnology 2015-08, Vol.24 (4), p.1413-1420
Main Authors: Yang, Hee Sun, Choi, Yu Jin, Jo, Jun Hee, Lee, Sang Cheon, Kim, Kyung Je, Jin, Seong Woo, Park, Tea Young, Huh, Chang Ki
Format: Article
Language:English
Subjects:
Bacteria
Bifidobacterium bifidum
Ganoderma lucidum
Lactobacillus sakei
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Summary:The purpose of this study was to elucidate the neuroprotective activities of Ganoderma lucidum water extract (GW) and fermented G. lucidum water extract (FGW). GW was fermented by the lactic acid bacterium Bifidobacterium bifidum (FGWB), followed by Lactobacillus sakei subsp. sakei LI033 (FGWBL). GW and FGW inhibited acetylcholinesterase (AChE) activity in a dose-dependent manner. In terms of cell viability and lactate dehydrogenase (LDH) release, GW and FGW showed neuroprotective activities against H sub(2)O sub(2)-stimulated oxidative stress in PC12 cells. Further, GW and FGW reduced H sub(2)O sub(2)-stimulated apoptosis, as determined by Hoechst staining. The neuroprotective effects of GW and FGW were found to be caspase-dependent based on reduction of caspase-3 activity and increased cell viability after caspase inhibitor (z-VAD-fmk) treatment. Taken together, these results suggest that GW and FGW may be useful in reducing risk of neurodegenerative diseases.
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-015-0181-1