In vitro polyploidy induction: changes in morphological, anatomical and phytochemical characteristics of Thymus persicus (Lamiaceae)

Thymus persicus, which grows in the northwest area of Iran, is a valuable natural source of medicinal pentacyclic triterpenoids (PTs), i.e. betulinic acid, oleanolic acid and ursolic acid. An in vitro approach to polyploidy induction in the plant was examined for the first time. Polyploidization was...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2015-09, Vol.122 (3), p.573-583
Main Authors: Tavan, Mansoureh, Mirjalili, Mohammad Hossein, Karimzadeh, Ghasem
Format: Article
Language:English
Subjects:
Betulinic acid
Biomedical and Life Sciences
breeding
Chromosome number
Chromosomes
Colchicine
Deoxyribonucleic acid
Diploids
diploidy
DNA
Flow cytometry
Lamiaceae
Leaves
Life Sciences
medicinal properties
Metaphase
mixoploidy
Oleanolic acid
Original Paper
Pentacyclic triterpenoids
Physical characteristics
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
plantlets
Ploidy
Polyploidy
roots
stems
Stomata
tetraploidy
Thymus persicus
Triterpenoids
Ursolic acid
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Summary:Thymus persicus, which grows in the northwest area of Iran, is a valuable natural source of medicinal pentacyclic triterpenoids (PTs), i.e. betulinic acid, oleanolic acid and ursolic acid. An in vitro approach to polyploidy induction in the plant was examined for the first time. Polyploidization was induced using in vitro-grown T. persicus shoot-tips immersed in colchicine (0, 0.05, 0.1, 0.3 and 0.5 %) for 12–48 h. Ploidy levels of regenerates were determined by either flow cytometry or chromosome counting at the metaphase. The results confirmed that the mother diploid plant contained a chromosome number of 2n = 2x = 28, 2C DNA = 1.20 pg, whereas 2n = 4x = 56, 2C DNA = 2.39 pg was detected in the induced autotetraploids (CV% 
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-015-0789-0