Evaluation of EPS extraction protocols from anaerobic sludge for gel-based proteomic studies

Despite the importance of anaerobic sludge extracellular polymeric substances (EPSs), their characterization is limited to information regarding their chemical classes and molecular size. This work explores the possibility of using proteomic techniques to study the proteins present in this matrix. T...

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Bibliographic Details
Published in:Water science and technology 2015-01, Vol.72 (4), p.535-542
Main Authors: Zorel, J A, Aquino, S F, Sanson, A L, Castro-Borges, W, Silva, S Q
Format: Article
Language:English
Subjects:
Ammonium
Ammonium compounds
Ammonium sulfate
Anaerobiosis
Analytical methods
Biofilms
Biotechnology
Carbohydrates
Carbohydrates - analysis
Caustic soda
Chromatography
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Evaluation
Extracellular
Extraction
Gel electrophoresis
Gels
Heating
Molecular Weight
Polymers
Polymers - analysis
Proteins
Proteins - analysis
Proteomics
Proteomics - methods
Sewage - analysis
Size distribution
Size exclusion chromatography
Sludge
Sodium
Sodium dodecyl sulfate
Sodium lauryl sulfate
Studies
Sulfates
Tandem Mass Spectrometry
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Summary:Despite the importance of anaerobic sludge extracellular polymeric substances (EPSs), their characterization is limited to information regarding their chemical classes and molecular size. This work explores the possibility of using proteomic techniques to study the proteins present in this matrix. Thus, this paper compares eight EPS extraction methods regarding extraction yield, protein/carbohydrate ratio, size distribution profile and suitability to sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses. Despite the differences found in quantification and size exclusion chromatography assays, the band profile found for all methods was very similar. Considering the band pattern, extraction time and background level, heating method followed by ammonium sulfate precipitation proved to be the most appropriate method for gel-based analyses of anaerobic sludge EPS proteins.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2015.244