Dehydration of corneal anterior donor tissue with polyethylene glycol (PEG)-enriched media

• Published in: Cell and tissue banking 2015-09, Vol.16 (3), p.399-409
• Main Authors: Lie, Jessica T., Monnereau, Claire, Groeneveld-van Beek, Esther A., van der Wees, Jacqueline, Frank, Johannes, Bruinsma, Marieke, Melles, Gerrit R. J.
• Format: Article
• Language: English
• Subjects: Absorption, Physicochemical; Aged; Biomedical and Life Sciences; Biomedicine; Body Water - chemistry; Cell Biology; Cornea; Corneal Transplantation; Dehydration; Desiccation - methods; Epithelium, Corneal - chemistry; Female; Humans; Life Sciences; Male; Organ Culture Techniques - methods; Organ Preservation - methods; Organ Preservation Solutions - chemistry; Polyethylene glycol; Polyethylene Glycols - chemistry; Skin & tissue grafts; Tissue Donors; Transplant Surgery
• ISSN: 1389-9333; 1573-6814
• DOI: 10.1007/s10561-014-9484-3

Anterior donor grafts (including scleral rim, without Descemet membrane) increase in thickness and become hazy upon storage in organ culture (OC) medium. Transfer of these grafts to standard dehydration media just before transplantation does not reduce their thickness to normal. Therefore, we assessed the efficacy of different media enriched with polyethylene glycol (PEG) as dehydrating agents for organ-cultured anterior donor grafts. Grafts were harvested and stored in the commercial OC medium ‘Max’ (without dextran) for 1 week, and subsequently dehydrated in the standard commercial dehydration medium ‘Jet’ (with dextran) supplemented with 4–20 % PEG3350, or ‘Max’ supplemented with 20 % PEG6000 and PEG20.000, or 5–20 % PEG35.000. Central corneal thickness (CCT), as assessed by anterior segment-optical coherence tomography, and transparency were evaluated before, and at 1, 4 and 7 days of dehydration. Transfer of grafts after 1 week of OC (average 1,200 µm) to ‘Jet’ supplemented with PEG3350 revealed a concentration-dependent effect of dehydration; CCT was restored to normal (500–600 µm) when 10 % PEG3350 was added. However, transparency was only temporarily restored; after 1 day, the grafts turned hazy. In contrast, grafts transferred to ‘Max’ supplemented with 20 % PEG35.000 were transparent throughout the evaluation period, but were dehydrated to beyond normal levels (average 300 µm). ‘Max’ supplemented with 5 % PEG35.000 dehydrated grafts to normal values and restored transparency throughout. Thus, dehydration of anterior donor grafts prior to surgery in dextran-free OC medium supplemented with 5 % PEG35.000 reduces graft thickness to normal and may facilitate anterior keratoplasty procedures.