Negative effect of combined cysteine and glutathione in soy lecithin-based extender on post-thawed ram spermatozoa

This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejacu...

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Bibliographic Details
Published in:Cell and tissue banking 2015-09, Vol.16 (3), p.443-448
Main Authors: Zhandi, Mahdi, Sharafi, Mohsen
Format: Article
Language:English
Subjects:
Amino acids
Animals
Antioxidants
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Movement - drug effects
Cell Movement - physiology
Cells, Cultured
Cryopreservation - methods
Cryoprotective Agents - administration & dosage
Cysteine - administration & dosage
Drug Combinations
Freezing
Glutathione
Lecithins - administration & dosage
Life Sciences
Male
Reproductive technologies
Rewarming
Semen Analysis
Sheep
Soybean Proteins - administration & dosage
Sperm
Spermatozoa - cytology
Spermatozoa - drug effects
Spermatozoa - physiology
Transplant Surgery
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Summary:This study was conducted to evaluate the effect of combined cysteine and glutathione in soy lecithin-based semen extender on post-thawed ram sperm quality. A total of 28 ejaculates were collected twice a week (from four rams) during breeding season. In each replicate, semen samples (n = 4, one ejaculate for each ram) were pooled and divided into three equal parts, and each part was diluted with one of following extender: (1) soy lecithin-based extender containing no cysteine and no glutathione (C0–G0), (2) soy lecithin-based extender containing cysteine (5 mM) and glutathione (5 mM) (C5–G5), and (3) soy lecithin-based extender containing cysteine (10 mM) and glutathione (10 mM) (C10–G10). After freeze–thawing process, motility and velocity parameters, plasma membrane integrity and functionality, mitochondrial activity, and apoptosis features of spermatozoa were evaluated. The obtained results showed that total and progressive motility, plasma membrane integrity and functionality, and live post-thawed spermatozoa was lower in C10–G10 extender compared to C0–G0 and C5–G5 extenders ( P  
ISSN:1389-9333
1573-6814
DOI:10.1007/s10561-014-9488-z