Purification and Identification of Two Antifungal Cyclic Peptides Produced by Bacillus amyloliquefaciens L-H15

Bacillus amyloliquefaciens L-H15 with broad spectrum antifungal activity was used as a biocontrol agent to suppress Fusarium oxysporum and other soil-borne fungal plant pathogens. Two antifungal fractions were isolated by bioactivity-guided reversed-phase high-performance liquid chromatography. The...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2015-08, Vol.176 (8), p.2202-2212
Main Authors: Han, Yuzhu, Zhang, Bao, Shen, Qian, You, Chengzhen, Yu, Yaqiong, Li, Pinglan, Shang, Qingmao
Format: Article
Language:English
Subjects:
Antifungal Agents - chemistry
Antifungal Agents - isolation & purification
Antifungal Agents - pharmacology
antifungal properties
antimicrobial peptides
Bacillus - chemistry
Bacillus amyloliquefaciens
Bacteria
Biochemistry
biocontrol
Biological control
biological control agents
Biotechnology
Chemistry
Chemistry and Materials Science
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Chromatography, Thin Layer
Fungi - drug effects
Fusarium oxysporum
iturin
Liquid chromatography
mass spectrometry
Mass spectroscopy
Microbial Sensitivity Tests
molecular weight
Pathogens
Peptides
Peptides, Cyclic - chemistry
Peptides, Cyclic - isolation & purification
Peptides, Cyclic - pharmacology
Plant diseases
plant diseases and disorders
plant pathogenic fungi
reversed-phase high performance liquid chromatography
Soil microorganisms
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sustainable agriculture
Tandem Mass Spectrometry
Ultrafiltration
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Summary:Bacillus amyloliquefaciens L-H15 with broad spectrum antifungal activity was used as a biocontrol agent to suppress Fusarium oxysporum and other soil-borne fungal plant pathogens. Two antifungal fractions were isolated by bioactivity-guided reversed-phase high-performance liquid chromatography. The two compounds were identified by tandem Q-TOF mass spectroscopy as C15 Iturin A (1) and a novel cyclic peptide with a molecular weight of 852.4 Da (2). Both compounds showed good inhibitory activities against three plant fungal pathogens in cylinder-plate diffusion assay. To our best knowledge, this is the first report on a cyclic antifungal peptide with a molecular weight of 852.4 Da. The strong antifungal activity suggests that the B. amyloliquefaciens L-H15 and its bioactive components might provide an alternative resource for the biocontrol of plant diseases and sustainable agriculture.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-015-1708-x