Expression of Finger Millet EcDehydrin7 in Transgenic Tobacco Confers Tolerance to Drought Stress

• Published in: Applied biochemistry and biotechnology 2015-09, Vol.177 (1), p.207-216
• Main Authors: Singh, Rajiv Kumar, Singh, Vivek Kumar, Raghavendrarao, Sanagala, Phanindra, Mullapudi Lakshmi Venkata, Venkat Raman, K., Solanke, Amolkumar U., Kumar, Polumetla Ananda, Sharma, Tilak Raj
• Format: Article
• Language: English
• Subjects: Abiotic stress; Adaptation, Physiological; Biochemistry; Biotechnology; Chemistry; Chemistry and Materials Science; Chlorophyll - metabolism; Climate change; Cloning, Molecular; Crops; Dehydration; Drought; Drought resistance; Droughts; Eleusine - metabolism; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Plant; Genetic Vectors - metabolism; Germination; Global warming; Millet; Nicotiana - genetics; Nicotiana - physiology; Plant breeding; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified; Seed germination; Seeds - growth & development; Sequence Analysis, DNA; Stress, Physiological; Sustainable agriculture; Tobacco; Transformation, Genetic; Transgenic plants; Water scarcity
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-015-1738-4

One of the critical alarming constraints for agriculture is water scarcity. In the current scenario, global warming due to climate change and unpredictable rainfall, drought is going to be a master player and possess a big threat to stagnating gene pool of staple food crops. So it is necessary to understand the mechanisms that enable the plants to cope with drought stress. In this study, effort was made to prospect the role of EcDehydrin7 protein from normalized cDNA library of drought tolerance finger millet in transgenic tobacco. Biochemical and molecular analyses of T 0 transgenic plants were done for stress tolerance. Leaf disc assay, seed germination test, dehydration assay, and chlorophyll estimation showed EcDehydrin7 protein directly link to drought tolerance. Northern and qRT PCR analyses shows relatively high expression of EcDehydrin7 protein compare to wild type. T 0 transgenic lines EcDehydrin7 (11) and EcDehydrin7 (15) shows superior expression among all lines under study. In summary, all results suggest that EcDehydrin7 protein has a remarkable role in drought tolerance and may be used for sustainable crop breeding program in other food crops.