Dependence of the bystander effect for micronucleus formation on dose of heavy-ion radiation in normal human fibroblasts

• Published in: Radiation protection dosimetry 2015-09, Vol.166 (1-4), p.152-156
• Main Authors: Matsumoto, Yoshitaka, Hamada, Nobuyuki, Aoki-Nakano, Mizuho, Funayama, Tomoo, Sakashita, Tetsuya, Wada, Seiichi, Kakizaki, Takehiko, Kobayashi, Yasuhiko, Furusawa, Yoshiya
• Format: Article
• Language: English
• Subjects: Bystander Effect - radiation effects; Cells, Cultured; Fibroblasts - cytology; Fibroblasts - physiology; Fibroblasts - radiation effects; Heavy Ions - adverse effects; Humans; Linear Energy Transfer - radiation effects; Micronuclei, Chromosome-Defective - radiation effects; Radiation Dosage
• ISSN: 0144-8420; 1742-3406
• DOI: 10.1093/rpd/ncv177

Ionising radiation-induced bystander effects are well recognised, but its dependence on dose or linear energy transfer (LET) is still a matter of debate. To test this, 49 sites in confluent cultures of AG01522D normal human fibroblasts were targeted with microbeams of carbon (103 keV µm(-1)), neon (375 keV µm(-1)) and argon ions (1260 keV µm(-1)) and evaluated for the bystander-induced formation of micronucleus that is a kind of a chromosome aberration. Targeted exposure to neon and argon ions significantly increased the micronucleus frequency in bystander cells to the similar extent irrespective of the particle numbers per site of 1-6. In contrast, the bystander micronucleus frequency increased with increasing the number of carbon-ion particles in a range between 1 and 3 particles per site and was similar in a range between 3 and 8 particles per site. These results suggest that the bystander effect of heavy ions for micronucleus formation depends on dose.