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Liquid Chromatography−Tandem Mass Spectrometry for the Determination of Protein-Bound Residues in Shrimp Dosed with Nitrofurans

An analytical method was developed for the determination of bound residues of the nitrofuran drugs furazolidone, nitrofurazone, furaltadone, and nitrofurantoin with a sensitivity of 1 ppb in shrimp. In this procedure, shrimp tissue is prewashed with solvents followed by overnight acid hydrolysis, du...

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Published in:	Journal of agricultural and food chemistry 2005-11, Vol.53 (23), p.8934-8939
Main Authors:	Chu, Pak-Sin, Lopez, Mayda I
Format:	Article
Language:	English
Subjects:	animal proteins Animals binding capacity Biological and medical sciences Chromatography, Liquid - methods drug residues Drug Residues - analysis Fish and seafood industries food analysis food contamination Food industries fualtadone Fundamental and applied biological sciences. Psychology furazolidone liquid chromatography Litopenaeus vannamei mass spectrometry Mass Spectrometry - methods nitrofurans Nitrofurans - administration & dosage Nitrofurans - analysis Nitrofurans - metabolism nitrofurantoin nitrofurazone Penaeidae - chemistry Proteins - metabolism seafoods shrimp
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description	An analytical method was developed for the determination of bound residues of the nitrofuran drugs furazolidone, nitrofurazone, furaltadone, and nitrofurantoin with a sensitivity of 1 ppb in shrimp. In this procedure, shrimp tissue is prewashed with solvents followed by overnight acid hydrolysis, during which the side chains of the bound residues are released and simultaneously derivatized with 2-nitrobenzaldehyde. After liquid−liquid extraction cleanup, the derivatives are detected and quantitated using liquid chromatography−mass spectrometry/mass spectrometry (LC-MS/MS) with an atmospheric pressure chemical ionization interface. The method was validated using control shrimp fortified with each side-chain analyte at 1, 2, and 4 ppb. Method accuracies were >80% with coefficients of variation of
doi_str_mv	10.1021/jf051615o
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In this procedure, shrimp tissue is prewashed with solvents followed by overnight acid hydrolysis, during which the side chains of the bound residues are released and simultaneously derivatized with 2-nitrobenzaldehyde. After liquid−liquid extraction cleanup, the derivatives are detected and quantitated using liquid chromatography−mass spectrometry/mass spectrometry (LC-MS/MS) with an atmospheric pressure chemical ionization interface. The method was validated using control shrimp fortified with each side-chain analyte at 1, 2, and 4 ppb. Method accuracies were >80% with coefficients of variation of <20% for all four analytes. Tissues from dosed shrimp were assayed to demonstrate the effectiveness of the method for recovering bound residues of nitrofurans. In shrimp dosed with nitrofurans, nitrofurantoin exhibited the lowest level of bound residues. Keywords: Nitrofurans; shrimp; LC-MS/MS; method; bound residues</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf051615o</identifier><identifier>PMID: 16277385</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>animal proteins ; Animals ; binding capacity ; Biological and medical sciences ; Chromatography, Liquid - methods ; drug residues ; Drug Residues - analysis ; Fish and seafood industries ; food analysis ; food contamination ; Food industries ; fualtadone ; Fundamental and applied biological sciences. Psychology ; furazolidone ; liquid chromatography ; Litopenaeus vannamei ; mass spectrometry ; Mass Spectrometry - methods ; nitrofurans ; Nitrofurans - administration & dosage ; Nitrofurans - analysis ; Nitrofurans - metabolism ; nitrofurantoin ; nitrofurazone ; Penaeidae - chemistry ; Proteins - metabolism ; seafoods ; shrimp</subject><ispartof>Journal of agricultural and food chemistry, 2005-11, Vol.53 (23), p.8934-8939</ispartof><rights>Copyright © 2005 American Chemical Society</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a436t-4e386712868d41ed7bdfe92481122f614a97e7d48b70c28c2e3c3ba3f4bfd5ad3</citedby><cites>FETCH-LOGICAL-a436t-4e386712868d41ed7bdfe92481122f614a97e7d48b70c28c2e3c3ba3f4bfd5ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17277487$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16277385$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chu, Pak-Sin</creatorcontrib><creatorcontrib>Lopez, Mayda I</creatorcontrib><title>Liquid Chromatography−Tandem Mass Spectrometry for the Determination of Protein-Bound Residues in Shrimp Dosed with Nitrofurans</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>An analytical method was developed for the determination of bound residues of the nitrofuran drugs furazolidone, nitrofurazone, furaltadone, and nitrofurantoin with a sensitivity of 1 ppb in shrimp. In this procedure, shrimp tissue is prewashed with solvents followed by overnight acid hydrolysis, during which the side chains of the bound residues are released and simultaneously derivatized with 2-nitrobenzaldehyde. After liquid−liquid extraction cleanup, the derivatives are detected and quantitated using liquid chromatography−mass spectrometry/mass spectrometry (LC-MS/MS) with an atmospheric pressure chemical ionization interface. The method was validated using control shrimp fortified with each side-chain analyte at 1, 2, and 4 ppb. Method accuracies were >80% with coefficients of variation of <20% for all four analytes. Tissues from dosed shrimp were assayed to demonstrate the effectiveness of the method for recovering bound residues of nitrofurans. In shrimp dosed with nitrofurans, nitrofurantoin exhibited the lowest level of bound residues. Keywords: Nitrofurans; shrimp; LC-MS/MS; method; bound residues</description><subject>animal proteins</subject><subject>Animals</subject><subject>binding capacity</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>drug residues</subject><subject>Drug Residues - analysis</subject><subject>Fish and seafood industries</subject><subject>food analysis</subject><subject>food contamination</subject><subject>Food industries</subject><subject>fualtadone</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>furazolidone</subject><subject>liquid chromatography</subject><subject>Litopenaeus vannamei</subject><subject>mass spectrometry</subject><subject>Mass Spectrometry - methods</subject><subject>nitrofurans</subject><subject>Nitrofurans - administration & dosage</subject><subject>Nitrofurans - analysis</subject><subject>Nitrofurans - metabolism</subject><subject>nitrofurantoin</subject><subject>nitrofurazone</subject><subject>Penaeidae - chemistry</subject><subject>Proteins - metabolism</subject><subject>seafoods</subject><subject>shrimp</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpt0MFuEzEQBuAVAtFQOPAC4AtIHBY89u7aPTYppUgBCkm5Ws563Dhk16ntVZsjN848Ik-CUaLmwsmH-fR75i-K50DfAmXwbmVpDQ3U_kExgprRsgaQD4sRzcNS1g0cFU9iXFFKZS3o4-IIGiYEl_Wo-Dl1N4MzZLIMvtPJXwe9WW7__Po9173BjnzSMZLZBtuU55jCllgfSFoiOcOEoXO9Ts73xFtyGXxC15djP_SGfMPozICRuJ7MlsF1G3LmIxpy69KSfHY5zw5B9_Fp8cjqdcRn-_e4uDp_P59clNMvHz5OTqelrniTygq5bAQw2UhTARqxMBZPWCUBGLMNVPpEoDCVXAjaMtky5C1faG6rhTW1Nvy4eL3L3QR_kxdLqnOxxfVa9-iHqEAAZ0Blhm92sA0-xoBWbfL6OmwVUPWvb3Xfd7Yv9qHDokNzkPuCM3i1Bzq2em3zxa2LByeyq6TIrtw5FxPe3c91-KEawUWt5pczdT5uxl-_w0RdZP9y5632Sl-HnHk1YxQ4BVpxSpvDz7qNauWH0Od2_3PCX8vbrx8</recordid><startdate>20051116</startdate><enddate>20051116</enddate><creator>Chu, Pak-Sin</creator><creator>Lopez, Mayda I</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20051116</creationdate><title>Liquid Chromatography−Tandem Mass Spectrometry for the Determination of Protein-Bound Residues in Shrimp Dosed with Nitrofurans</title><author>Chu, Pak-Sin ; Lopez, Mayda I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a436t-4e386712868d41ed7bdfe92481122f614a97e7d48b70c28c2e3c3ba3f4bfd5ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>animal proteins</topic><topic>Animals</topic><topic>binding capacity</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>drug residues</topic><topic>Drug Residues - analysis</topic><topic>Fish and seafood industries</topic><topic>food analysis</topic><topic>food contamination</topic><topic>Food industries</topic><topic>fualtadone</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>furazolidone</topic><topic>liquid chromatography</topic><topic>Litopenaeus vannamei</topic><topic>mass spectrometry</topic><topic>Mass Spectrometry - methods</topic><topic>nitrofurans</topic><topic>Nitrofurans - administration & dosage</topic><topic>Nitrofurans - analysis</topic><topic>Nitrofurans - metabolism</topic><topic>nitrofurantoin</topic><topic>nitrofurazone</topic><topic>Penaeidae - chemistry</topic><topic>Proteins - metabolism</topic><topic>seafoods</topic><topic>shrimp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chu, Pak-Sin</creatorcontrib><creatorcontrib>Lopez, Mayda I</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chu, Pak-Sin</au><au>Lopez, Mayda I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid Chromatography−Tandem Mass Spectrometry for the Determination of Protein-Bound Residues in Shrimp Dosed with Nitrofurans</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2005-11-16</date><risdate>2005</risdate><volume>53</volume><issue>23</issue><spage>8934</spage><epage>8939</epage><pages>8934-8939</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>An analytical method was developed for the determination of bound residues of the nitrofuran drugs furazolidone, nitrofurazone, furaltadone, and nitrofurantoin with a sensitivity of 1 ppb in shrimp. In this procedure, shrimp tissue is prewashed with solvents followed by overnight acid hydrolysis, during which the side chains of the bound residues are released and simultaneously derivatized with 2-nitrobenzaldehyde. After liquid−liquid extraction cleanup, the derivatives are detected and quantitated using liquid chromatography−mass spectrometry/mass spectrometry (LC-MS/MS) with an atmospheric pressure chemical ionization interface. The method was validated using control shrimp fortified with each side-chain analyte at 1, 2, and 4 ppb. Method accuracies were >80% with coefficients of variation of <20% for all four analytes. Tissues from dosed shrimp were assayed to demonstrate the effectiveness of the method for recovering bound residues of nitrofurans. In shrimp dosed with nitrofurans, nitrofurantoin exhibited the lowest level of bound residues. Keywords: Nitrofurans; shrimp; LC-MS/MS; method; bound residues</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16277385</pmid><doi>10.1021/jf051615o</doi><tpages>6</tpages></addata></record>
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source	American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects	animal proteins Animals binding capacity Biological and medical sciences Chromatography, Liquid - methods drug residues Drug Residues - analysis Fish and seafood industries food analysis food contamination Food industries fualtadone Fundamental and applied biological sciences. Psychology furazolidone liquid chromatography Litopenaeus vannamei mass spectrometry Mass Spectrometry - methods nitrofurans Nitrofurans - administration & dosage Nitrofurans - analysis Nitrofurans - metabolism nitrofurantoin nitrofurazone Penaeidae - chemistry Proteins - metabolism seafoods shrimp
title	Liquid Chromatography−Tandem Mass Spectrometry for the Determination of Protein-Bound Residues in Shrimp Dosed with Nitrofurans
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