Induction by alkaloids and phenobarbital of Family 4 Cytochrome P450s in Drosophila: evidence for involvement in host plant utilization

In vertebrates, cytochrome P450s of the CYP2 and CYP3 families play a dominant role in drug metabolism, while in insects members of the CYP6 and CYP28 families have been implicated in metabolism of insecticides and toxic natural plant compounds. A degenerate 3' RACE strategy resulted in the ide...

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Bibliographic Details
Published in:Molecular & general genetics 1998-07, Vol.259 (1), p.54-59
Main Authors: Danielson, P B, Foster, J L, McMahill, M M, Smith, M K, Fogleman, J C
Format: Article
Language:English
Subjects:
Alkaloids - pharmacology
Animals
Blotting, Northern
Cytochrome P-450 Enzyme System - biosynthesis
Cytochrome P-450 Enzyme System - drug effects
Cytochrome P-450 Enzyme System - genetics
DNA, Complementary - isolation & purification
Drosophila - drug effects
Drosophila - enzymology
Drosophila - genetics
Drosophila mettleri
Drosophila Proteins
Enzyme Induction - drug effects
Enzyme Induction - genetics
Female
Molecular Sequence Data
Phenobarbital - pharmacology
Phylogeny
Plants - enzymology
Plants - metabolism
Polymerase Chain Reaction
Transcription, Genetic - drug effects
Xenobiotics - pharmacology
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Summary:In vertebrates, cytochrome P450s of the CYP2 and CYP3 families play a dominant role in drug metabolism, while in insects members of the CYP6 and CYP28 families have been implicated in metabolism of insecticides and toxic natural plant compounds. A degenerate 3' RACE strategy resulted in the identification of fifteen novel P450s from an alkaloid-resistant species of Drosophila. The strong (17.4-fold) and highly specific induction of a single gene (CYP4D10) by the toxic isoquinoline alkaloids of a commonly utilized host-plant (saguaro cactus) provides the first indication that members of the CYP4 family in insects may play an important role in the maintenance of specific insect-host plant relationships. Strong barbiturate inducibility of CYP4D10 and two other D. mettleri P450 sequences of the CYP4 family was also observed, suggesting a pattern of xenobiotic responsiveness more similar to those of several vertebrate drug-metabolizing enzymes than to putative vertebrate CYP4 homologs.
ISSN:0026-8925
1432-1874
DOI:10.1007/s004380050788