Chicken anemia virus strains with a mutated enhancer/promoter region share reduced virus spread and cytopathogenicity

Plasmid pCAV/E contains an infectious cloned double-stranded CAV (chicken anemia virus) DNA genome (Noteborn et al., J. Virol. 65 (1991) 3131–3139). We have constructed mutated CAV genomes by introducing mutations into the CAV promoter/enhancer region of pCAV/E. Various mutated CAV strains were func...

Full description

Saved in:
Bibliographic Details
Published in:Gene 1998-11, Vol.223 (1-2), p.165-172
Main Authors: Noteborn, Mathieu H.M., Verschueren, Claudia A.J., van Ormondt, Hans, van der Eb, Alex J.
Format: Article
Language:English
Subjects:
12-bp-insert promoter element
Animals
Attenuation
Cells, Cultured - virology
Chicken anemia virus
Chicken anemia virus - genetics
Chicken anemia virus - immunology
Chicken anemia virus - pathogenicity
Chickens
cytopathogenicity
DNA
dna replication intermediates
Enhancer Elements, Genetic
enhancer sequences
Epitopes
Genome, Viral
mutagenesis
mutants
Mutation
neutralizing antibodies
neutralizing epitopes
plasmid vectors
promoter regions
Promoter Regions, Genetic
T-lymphocytes
T-Lymphocytes - virology
transfection
vaccine development
Vaccine prototype
viral antigens
Virus replication
Virus Replication - genetics
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plasmid pCAV/E contains an infectious cloned double-stranded CAV (chicken anemia virus) DNA genome (Noteborn et al., J. Virol. 65 (1991) 3131–3139). We have constructed mutated CAV genomes by introducing mutations into the CAV promoter/enhancer region of pCAV/E. Various mutated CAV strains were functional and had a smaller cytopathogenic effect in chicken T cells than wild-type CAV. In particular, mutations within the `12-bp insert' of the promoter/enhancer region had this effect. PCR and sequence analysis showed that the CAV mutants were stable under cell-culture conditions. Southern-blot analysis showed that all replication DNA intermediates were normally formed by the CAV mutants. All viable mutant CAV strains were able to produce a neutralizing conformational epitope, which implies that they can trigger the required protective immune response. These features make these mutant CAV strains potential candidates for the development of an attenuated CAV vaccine.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(98)00170-X