Protection against anthrax toxin by vaccination with a DNA plasmid encoding anthrax protective antigen

A DNA vaccine encoding the immunogenic and biologically active portion of anthrax protective antigen (PA) was constructed. Spleen cells from BALB/c mice immunized intramuscularly with this vaccine were stimulated to secrete IFN γ and IL-4 when exposed to PA in vitro. Immunized mice also mounted a hu...

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Bibliographic Details
Published in:Vaccine 1999-01, Vol.17 (4), p.340-344
Main Authors: Gu, Mi-Li, Leppla, Stephen H, Klinman, Dennis M
Format: Article
Language:English
Subjects:
Animals
Anthrax
Antibodies, Bacterial - biosynthesis
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Bacillus anthracis - immunology
Bacterial Toxins - immunology
Bacteriology
Biological and medical sciences
DNA - genetics
DNA vaccine
Female
Fundamental and applied biological sciences. Psychology
Genetic Code
Interferon-gamma - metabolism
Interleukin-4 - metabolism
Lethal Dose 50
Mice
Mice, Inbred BALB C
Microbiology
Plasmids - genetics
Toxin
Vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:A DNA vaccine encoding the immunogenic and biologically active portion of anthrax protective antigen (PA) was constructed. Spleen cells from BALB/c mice immunized intramuscularly with this vaccine were stimulated to secrete IFN γ and IL-4 when exposed to PA in vitro. Immunized mice also mounted a humoral immune response dominated by IgG1 anti-PA antibody production, the subclass previously shown to confer protection against anthrax toxin. A 1:100 dilution of serum from these animals protected cells in vitro against cytotoxic concentrations of PA. Moreover, 7/8 mice immunized three times with the PA DNA vaccine were protected against lethal challenge with a combination of anthrax protective antigen plus lethal factor.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(98)00210-2