Application of Minimal Sequence Quality Values Prevents Misidentification of the bla sub(SHV) Type in Single Bacterial Isolates Carrying Different SHV Extended-Spectrum beta -Lactamase Genes

Nucleotide sequencing is the standard molecular method for determination of the beta -lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla sub(SHV) genes, as illustrated by three strains of three different species that each contained two diffe...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2006-05, Vol.44 (5), p.1896-1898
Main Authors: al Naimi, Nashwan, Schipper, Kim, Duim, Birgitta, Bart, Aldert
Format: Article
Language:English
Subjects:
Bacteria
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Summary:Nucleotide sequencing is the standard molecular method for determination of the beta -lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla sub(SHV) genes, as illustrated by three strains of three different species that each contained two different bla sub(SHV) alleles, SHV-2 and SHV-12.
ISSN:0095-1137
1098-5530