Imprinted expression and methylation of the mouse H19 gene are conserved in extraembryonic lineages

The imprinted H19 gene is hypomethylated on the active maternal allele and hypermethylated on the repressed paternal allele in the somatic tissues of mice and humans. We previously demonstrated that the paternal‐specific methylation of a 2 kb region located between −2 and −4 kb relative to the start...

Full description

Saved in:
Bibliographic Details
Published in:Developmental genetics 1998, Vol.23 (2), p.111-118
Main Authors: Davis, Tamara L., Tremblay, Kimberly D., Bartolomei, Marisa S.
Format: Article
Language:English
Subjects:
Alleles
Animals
Bisulfite mutagenesis
Cell Lineage
Crosses, Genetic
DNA Methylation
extraembryonic lineages
Female
Gene Expression Regulation, Developmental
Genomic Imprinting - genetics
H19
imprinting
Male
Mice
Muscle Proteins - biosynthesis
Muscle Proteins - genetics
Mutagenesis
Organ Specificity
RNA, Long Noncoding
RNA, Untranslated
Yolk Sac - chemistry
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The imprinted H19 gene is hypomethylated on the active maternal allele and hypermethylated on the repressed paternal allele in the somatic tissues of mice and humans. We previously demonstrated that the paternal‐specific methylation of a 2 kb region located between −2 and −4 kb relative to the start of transcription is maintained throughout murine development, and we thus propose that this region is crucial to determining the imprinted expression of H19. Here, we test the correlation between differential methylation and imprinted expression by analyzing the mouse H19 gene in the undermethylated extraembryonic tissues. During early and midpostimplantation stages, >95% of the H19 RNA is derived from the maternal allele. Dissection of yolk sac revealed that the paternal allele is expressed at a low level in the viseral endoderm but is completely repressed in visceral mesoderm. Bisulfite methylation analysis of yolk sac DNA showed that the maternal allele was hypomethylated and that 95% of the paternally derived clones were hypermethylated. Thus in extraembryonic lineages, the majority of H19 DNA is differentially methylated. These results lend further support to the hypothesis that DNA methylation confers the imprint on H19. Dev. Genet. 23:111–118, 1998. © 1998 Wiley‐Liss, Inc.
ISSN:0192-253X
1520-6408
DOI:10.1002/(SICI)1520-6408(1998)23:2<111::AID-DVG3>3.0.CO;2-9