An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device

We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this...

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Bibliographic Details
Published in:Transgenic research 1998-07, Vol.7 (4), p.289-294
Main Authors: DURAILAGARAJA SUDHAKAR, LE TAN DUC, BUI BA BONG, PORNTIP TINJUANGJUN, SHAHINA BANO MAQBOOL, VALDEZ, M, JEFFERSON, R, CHRISTOU, P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Oryza sativa
Rice
Transgenic animals and transgenic plants
Transgenic plants
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Summary:We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.[PUBLICATION ABSTRACT]
ISSN:0962-8819
1573-9368
DOI:10.1023/A:1008870012568