In vitro isolation and characterisation of the first canine Neospora caninum isolate in Australia

Neospora caninum was isolated and established in vitro from the skin lesion of a naturally infected dog. The identity of the parasite was evaluated by immunofluorescent antibody test (IFAT), microscopy, Western blotting and polymerase chain reaction (PCR). N. caninum DNA was detected in the whole bl...

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Bibliographic Details
Published in:Veterinary parasitology 2006-04, Vol.137 (3), p.355-363
Main Authors: McInnes, L.M., Irwin, P., Palmer, D.G., Ryan, U.M.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Protozoan - blood
Antiprotozoal Agents - therapeutic use
Australia
Base Sequence
Blotting, Western - veterinary
Coccidiosis - drug therapy
Coccidiosis - parasitology
Coccidiosis - veterinary
Cutaneous lesions
DNA, Protozoan - analysis
Dog
Dog Diseases - drug therapy
Dog Diseases - parasitology
Dogs
Female
fluorescent antibody technique
Fluorescent Antibody Technique, Indirect - veterinary
Gene Amplification
genes
genetic variation
IFAT
in vitro culture
isolation
Neospora - classification
Neospora - immunology
Neospora - isolation & purification
Neospora - ultrastructure
Neospora caninum
neosporosis
nucleotide sequences
pathogen identification
PCR
Phylogeny
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
sequence analysis
skin lesions
strain differences
Strain variation
Treatment Outcome
Western blotting
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Description
Summary:Neospora caninum was isolated and established in vitro from the skin lesion of a naturally infected dog. The identity of the parasite was evaluated by immunofluorescent antibody test (IFAT), microscopy, Western blotting and polymerase chain reaction (PCR). N. caninum DNA was detected in the whole blood, serum, skin lesion, rectal scrapings and faeces of the infected dog utilising a nested PCR targeting the Nc-5 gene of N. caninum. Antigenic and genetic characterisation of the isolate, designated WA-K9, at a number of loci including the Nc-5 gene, heat shock protein 70 (HSP-70) gene, alpha-tubulin and beta-tubulin genes revealed no variation between this isolate and two N. caninum isolates from different geographic areas. Clinical aspects of this case, which included cutaneous and neurological disease, are also discussed.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2006.01.018