A rapid method for the identification and partial serotyping of Listeria monocytogenes in food by PCR and restriction enzyme analysis

Two highly specific primers for Listeria monocytogenes were used to yield from foods such as milk, soft cheese and meat, PCR products that were cleaved with the restriction enzyme HindIII. The fragments generated allowed a distinction between two groups of L. monocytogenes serovars: serovars 1/2a an...

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Bibliographic Details
Published in:International journal of food microbiology 1998-07, Vol.42 (3), p.207-212
Main Authors: Manzano, Marisa, Cocolin, Luca, Cantoni, Carlo, Comi, Giuseppe
Format: Article
Language:English
Subjects:
ALIMENTOS
Animals
Biological and medical sciences
Cattle
DNA Primers - chemistry
DNA Restriction Enzymes - chemistry
DNA, Bacterial - chemistry
Electrophoresis, Agar Gel
Food industries
Food Microbiology
FOODS
Fundamental and applied biological sciences. Psychology
Humans
iap gene
IDENTIFICACION
IDENTIFICATION
LISTERIA MONOCYTOGENES
Listeria monocytogenes - chemistry
Listeria monocytogenes - classification
Listeria monocytogenes - genetics
Meat - microbiology
Milk - microbiology
PCR
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
PRODUIT ALIMENTAIRE
Restriction enzyme analysis
SEROTIPOS
SEROTYPE
SEROTYPES
Serotyping - methods
Swine
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Description
Summary:Two highly specific primers for Listeria monocytogenes were used to yield from foods such as milk, soft cheese and meat, PCR products that were cleaved with the restriction enzyme HindIII. The fragments generated allowed a distinction between two groups of L. monocytogenes serovars: serovars 1/2a and 1/2c cluster in one group and serovars 1/2b, 3b and 4b in the other subgroup. Since this procedure can be completed in 24 h, an epidemiological association between human disease and suspected sources can be rapidly confirmed at the subgroup level in the laboratory.
ISSN:0168-1605
1879-3460
DOI:10.1016/S0168-1605(98)00086-5