The effect of sugars and free amino acids from the freshwater prawn Macrobrachium rosenbergii hemolymph on lectin activity and on oxidative burst

We determined the effect of low molecular weight components (LMWC) from healthy juvenile and adult Macrobrachium rosenbergii hemolymph on lectin activity and oxidative burst (OB) in hemocytes. In an attempt to identify the LMWC that affect the lectin's hemagglutinating activity or oxidative bur...

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Bibliographic Details
Published in:Comparative biochemistry and physiology. Toxicology & pharmacology 2006-03, Vol.142 (3-4), p.212-219
Main Authors: Soria, Frida, Sierra, Claudia, Bouquelet, Stephane, Brassart, Colette, Agundis, Concepción, Zenteno, Edgar, Vázquez, Lorena
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - blood
Amino Acids - chemistry
Animals
Carbohydrates
Carbohydrates - blood
Carbohydrates - chemistry
Erythrocytes - physiology
Fresh Water
Glycine
Hemagglutination
Hemocytes - metabolism
Hemolymph - metabolism
Lectin
Lectins - metabolism
Macrobrachium rosenbergii
Oxidative burst
Palaemonidae - metabolism
Rats
Rats, Wistar
Respiratory Burst
Superoxides - metabolism
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Summary:We determined the effect of low molecular weight components (LMWC) from healthy juvenile and adult Macrobrachium rosenbergii hemolymph on lectin activity and oxidative burst (OB) in hemocytes. In an attempt to identify the LMWC that affect the lectin's hemagglutinating activity or oxidative burst, we determined the hemolymph carbohydrates and free amino acids (FAA) concentration. The LMWC (40%) and alanine (26%). N-Acetyl-d-glucosamine (GlcNAc) was the main sugar residue in the hemolymph and LMWC from juveniles; its concentration was 2.4 times higher than glucose (Glc), whereas, in adults, Glc was the main free sugar residue. Our results suggest that the proportion of FAA and carbohydrates in the hemolymph of M. rosenbergii seems to be correlated with the maturation process; furthermore, the high proportion of free GlcNAc and glycine regulate, in the juvenile stage, lectin activity and cellular oxidative mechanisms, respectively.
ISSN:1532-0456
1878-1659
DOI:10.1016/j.cbpc.2005.10.003