Establishment and characterization of SUIT-58 pancreas cancer cell line and its subline S58-SF adapted to serum-free condition derived from metastatic liver tumor

• Published in: Human cell : official journal of Human Cell Research Society 2015-10, Vol.28 (4), p.190-198
• Main Authors: Takahashi, Nobuyasu, Aoyama, Fumiyo, Ohuchida, Jiro, Sameshima, Naoki, Asada, Yujiro, Sawaguchi, Akira
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma - pathology; Adenocarcinoma - secondary; Adenocarcinoma - ultrastructure; Aged; Amino Acid Metabolism, Inborn Errors; Animals; Biomedical and Life Sciences; Cell Biology; Cell Culture Techniques - methods; Cell Line; Cell Line, Tumor; Cell Transformation, Neoplastic; Culture Media, Serum-Free; Dental Enamel Hypoplasia; Diabetes Mellitus; Dwarfism; Female; Gynecology; Heterografts; Humans; Intellectual Disability; Karyotyping; Life Sciences; Liver Neoplasms - genetics; Liver Neoplasms - pathology; Liver Neoplasms - secondary; Liver Neoplasms - ultrastructure; Mice, Nude; Microcephaly; Microscopy, Electrochemical, Scanning; Neoplasm Transplantation; Oncology; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - pathology; Pancreatic Neoplasms - ultrastructure; Reproductive Medicine; Reverse Transcriptase Polymerase Chain Reaction; Stem Cells; Surgery
• ISSN: 1749-0774; 1749-0774
• DOI: 10.1007/s13577-015-0122-6

A new pancreas cancer cell line, SUIT-58, was established from metastatic liver tumor. The cultured cells exhibited polygonal shape, and proliferated in a form of sheet-structure showing prominent nucleoli and frequent mitotic features. Chromosome count ranged from 54 to 73 with modal chromosome numbers 72 and 73. It was noteworthy that this cell line grew in the serum-free media and maintained in this condition for 30 passages (designated as S58-SF). Both SUIT-58 and S58-SF cell lines were successfully transplanted into nude mice, and their tumor doubling times in xenografts were calculated as 5.4 and 2.8 days, respectively. Histopathologically, the xenografts formed glandular structure that resembled the original tumor. In culture media, the doubling time of SUIT-58 and S58-SF cell lines was calculated as 32 and 35.7 h, respectively. Although the cellular arrangements of SUIT-58 and S58-SF cell lines are different to some extent, their subcellular structures under electron microscope were similar with a large number of lysosomes and distinct desmosomes at cell-cell adhesion sites. The present SUIT-58 and its derivative cell line S58-SF will be applicable for biological studies to develop a new clinical treatment of refractory pancreatic cancer.