Preferential type 1 chemokine receptors and cytokine production of CD28 super(-) T cells in ankylosing spondylitis

OBJECTIVE: To examine serum levels of type 1 and type 2 chemokines and lymphocytic expression of chemokine receptors, and to compare the results with lymphocytic cytokine production in patients with ankylosing spondylitis (AS). METHODS: Twelve patients with AS (mean (SD) age 44.9 (14.7) years) and 2...

Full description

Saved in:
Bibliographic Details
Published in:Annals of the rheumatic diseases 2006-05, Vol.65 (5), p.647-653
Main Authors: Duftner, C, Dejaco, C, Kullich, W, Klauser, A, Goldberger, C, Falkenbach, A, Schirmer, M
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:OBJECTIVE: To examine serum levels of type 1 and type 2 chemokines and lymphocytic expression of chemokine receptors, and to compare the results with lymphocytic cytokine production in patients with ankylosing spondylitis (AS). METHODS: Twelve patients with AS (mean (SD) age 44.9 (14.7) years) and 27 healthy controls (46.4 (12.8) years) were enrolled into the study. The expression of chemokine receptors (CCR-5, CXCR-3, CCR-4) and cytokines (interferon gamma (IFN gamma ), interleukin (IL)2, IL4, IL10, tumour necrosis factor alpha (TNF alpha )) on CD28 super(+) and CD28 super(-) T cell subtypes was analysed by a three colour FACS technique of peripheral blood samples. Serum ELISAs were performed to detect the CCR-5 ligands CCL-5, CCL-3; the CXCR-3 ligands CXCL-10, CXCL-9; and the CCR-4 ligand, CCL-17 before and after administration of the TNF alpha blocking agent infliximab. RESULTS: CD4 super(+)CD28 super(-) T cells had higher ratios of CXCR-3 to CCR-4 than CD4 super(+)CD28 super(+) T cells. Both, CD4 super(+) and CD8 super(+)CD28 super(-) T cells of patients with AS produced more IFN gamma , TNF alpha , and IL10 than their CD28 super(+) counterparts (p
ISSN:0003-4967