Elevated Levels of M 92,000 Type IV Collagenase during Tumor Growth in Vivo

Our previous studies demonstrated that matrix metalloproteinase (MMP-9) levels were significantly higher in human glioblastoma tissue samples than in low-grade brain tumors and normal brain tissue. In the present study, we measured the levels of MMP-2 and MMP-9 during the growth of glial tumors in n...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-10, Vol.251 (2), p.632-636
Main Authors: Sawaya, R, Go, Y, Kyritisis, A P, Uhm, J, Venkaiah, B, Mohanam, S, Gokaslan, Z L, Rao, J S
Format: Article
Language:English
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Summary:Our previous studies demonstrated that matrix metalloproteinase (MMP-9) levels were significantly higher in human glioblastoma tissue samples than in low-grade brain tumors and normal brain tissue. In the present study, we measured the levels of MMP-2 and MMP-9 during the growth of glial tumors in nude mice by intracerebral injection of glioblastoma cells. Using gelatin zymography, densitometry, and an enzyme-linked immunosorbent assay, we found that the enzyme activity and protein count of MMP-2 and MMP-9 were a respective 3- to 10- and 2- to 30-fold higher in tumors at day 14 and 28 than in normal tissue. Immunohistochemical staining for MMP-9 showed strong immunoreactivity in tumor cells and the staining intensity was much higher at day 28, compared to day 14. These results suggest that upregulation of MMP-9 plays a major role in the glioma tumor growth in vivo.
ISSN:0006-291X
DOI:10.1006/bbrc.1998.9466