Phylogeography of the arid-adapted Malagasy bullfrog, Laliostoma labrosum, influenced by past connectivity and habitat stability

• Published in: Molecular phylogenetics and evolution 2015-11, Vol.92, p.11-24
• Main Authors: Pabijan, Maciej, Brown, Jason L., Chan, Lauren M., Rakotondravony, Hery A., Raselimanana, Achille P., Yoder, Anne D., Glaw, Frank, Vences, Miguel
• Format: Article
• Language: English
• Subjects: Amphibia; Animals; Anura - classification; Anura - genetics; Anura - physiology; Biodiversity; Cell Nucleus - genetics; Desert Climate; DNA, Mitochondrial - genetics; Ecosystem; Female; Fresh Water - analysis; Gene Flow; Genetic Variation - genetics; Madagascar; Male; Multilocus; Phylogeny; Phylogeography; Population Density; Rainforest; RNA, Ribosomal, 16S - genetics; Seasons; Species distribution model
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2015.05.018

[Display omitted] •We examine the phylogeography of an amphibian (frog) from arid western Madagascar.•Low multilocus differentiation may be a consequence of high individual movement.•Sambirano rainforest constitutes a major phylogeographic barrier for this species.•Ancestral distributions of mtDNA clades suggest five potential Pleistocene refugia.•High regional population connectivity essential for long-term persistence. The rainforest biome of eastern Madagascar is renowned for its extraordinary biodiversity and restricted distribution ranges of many species, whereas the arid western region of the island is relatively species poor. We provide insight into the biogeography of western Madagascar by analyzing a multilocus phylogeographic dataset assembled for an amphibian, the widespread Malagasy bullfrog, Laliostoma labrosum. We find no cryptic species in L. labrosum (maximum 1.1% pairwise genetic distance between individuals in the 16S rRNA gene) attributable to considerable gene flow at the regional level as shown by genetic admixture in both mtDNA and three nuclear loci, especially in central Madagascar. Low breeding site fidelity, viewed as an adaptation to the unreliability of standing pools of freshwater in dry and seasonal environments, and a ubiquitous distribution within its range may underlie overall low genetic differentiation. Moreover, reductions in population size associated with periods of high aridity in western Madagascar may have purged DNA variation in this species. The mtDNA gene tree revealed seven major phylogroups within this species, five of which show mostly non-overlapping distributions. The nested positions of the northern and central mtDNA phylogroups imply a southwestern origin for all extant mtDNA lineages in L. labrosum. The current phylogeography of this species and paleo-distributions of major mtDNA lineages suggest five potential refugia in northern, western and southwestern Madagascar, likely the result of Pleistocene range fragmentation during drier and cooler climates. Lineage sorting in mtDNA and nuclear loci highlighted a main phylogeographic break between populations north and south of the Sambirano region, suggesting a role of the coastal Sambirano rainforest as a barrier to gene flow. Paleo-species distribution models and dispersal networks suggest that the persistence of some refugial populations was mainly determined by high population connectivity through space and time.