Effect of hexachlorocyclohexane (HCH), its isomers, and metabolites of Hsp70 expression in transgenic Drosophila melanogaster

Expression of hsp70 in the tissues of third-instar larvae of the Bg9 (hsp70-lacZ) strain of Drosophila melanogaster after exposure to hexachlorocyclohexane (HCH) (0.5, 1.0, 1.5, 2.0, and 5.0 ng/ml) in the diet was studied by histochemical staining for beta-galactosidase activity and puffing activity...

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Bibliographic Details
Published in:Pesticide biochemistry and physiology 1999, Vol.63 (1), p.15-25
Main Authors: Chowdhuri, D.K, Saxena, D.K, Viswanathan, P.N
Format: Article
Language:English
Subjects:
beta-galactosidase
Biological and medical sciences
cell biology
Chemical control
Control
Drosophila melanogaster
enzyme activity
Fundamental and applied biological sciences. Psychology
gene expression
HCH (pesticide)
heat shock proteins
histochemistry
isomers
larvae
lindane
metabolites
Phytopathology. Animal pests. Plant and forest protection
polytene chromosomes
Protozoa. Invertebrates
transgenic animals
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Summary:Expression of hsp70 in the tissues of third-instar larvae of the Bg9 (hsp70-lacZ) strain of Drosophila melanogaster after exposure to hexachlorocyclohexane (HCH) (0.5, 1.0, 1.5, 2.0, and 5.0 ng/ml) in the diet was studied by histochemical staining for beta-galactosidase activity and puffing activity at 9E, 87A, and 87C of the salivary gland polytene chromosome was measured. At concentrations of 2.0 and 5.0 ng/ml, the proventriculus of the foregut, gastric caeca (part or whole), brain ganglia, imaginal discs, salivary glands, midgut, and hindgut of the larvae showed beta-galactosidase activity. In controls and at dietary concentrations of 0.5 and 1.0 ng/ml, only the proventriculus and part of the salivary gland showed activity, while at 1.5 ng/ml, imaginal discs, the proximal part of brain ganglia, and the whole salivary gland showed positive staining. Among the four isomers of HCH tested, the gamma isomer stimulated the most widespread expression of hsp70 in larval tissues proventriculus, gastric caeca, part of brain ganglia, imaginal discs, salivary gland, midgut, and hindgut) followed by alpha (proventriculus and salivary gland) and then by the beta and delta isomers which produced weak responses in the proventriculus only. Of the metabolites examined in the present study, 2,4,6-trichlorophenol (TCP) evoked the greatest expression of hsp70 compared to the other isomer 2,3,5-TCP and 1,2,3,4-tetrachlorobenzene (TTCB). The puffing pattern in the salivary gland polytene chromosome was similar to the histochemical staining pattern. The present study suggests that there are sites in the larvae vulnerable to HCH, its isomers, and metabolites as evidenced by hsp70 expression.
ISSN:0048-3575
1095-9939
DOI:10.1006/pest.1998.2390