Association of overexpression of efflux pump genes with antibiotic resistance in Pseudomonas aeruginosa strains clinically isolated from urinary tract infection patients

There are several mechanisms for antibiotic-resistant Pseudomonas aeruginosa . The purpose of this study is to investigate the association between the expression of efflux pump-coding genes and antibiotic resistance in P. aeruginosa causing urinary tract infections (UTIs). We extracted the RNA from...

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Bibliographic Details
Published in:Journal of antibiotics 2015-09, Vol.68 (9), p.568-572
Main Authors: Shigemura, Katsumi, Osawa, Kayo, Kato, Ayaka, Tokimatsu, Issei, Arakawa, Soichi, Shirakawa, Toshiro, Fujisawa, Masato
Format: Article
Language:English
Subjects:
38
692/308/575
Aged
Aged, 80 and over
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Antibiotics
Bacteriology
Biomedical and Life Sciences
Bioorganic Chemistry
Cell division
Drug resistance
Drug Resistance, Bacterial - genetics
Female
Gene Expression Regulation, Bacterial
Genes, Bacterial - genetics
Genes, MDR - genetics
Humans
Life Sciences
Male
Medicinal Chemistry
Microbiology
Middle Aged
Multivariate Analysis
Organic Chemistry
original-article
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - isolation & purification
Pseudomonas Infections - microbiology
Reverse Transcriptase Polymerase Chain Reaction
Risk Factors
RNA, Bacterial
Urinary tract infections
Urinary Tract Infections - microbiology
Urogenital system
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Summary:There are several mechanisms for antibiotic-resistant Pseudomonas aeruginosa . The purpose of this study is to investigate the association between the expression of efflux pump-coding genes and antibiotic resistance in P. aeruginosa causing urinary tract infections (UTIs). We extracted the RNA from 105 clinical strains of P. aeruginosa isolated from UTI patients with full data on antibiotic MICs and assayed real-time quantitative reverse-transcription PCR. We investigated the gene expressions of four resistance nodulation cell division-type multi-drug efflux pump systems (MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY(-OprA)) and the correlation of the MICs of nine antibiotics, risk factors and antibiotic resistance-related genes with expressions of mexB , mexC , mexE and mexY . Multivariate statistical data demonstrated a significant relationship between increased expression of mexB or mexC and complicated UTI (Odds ratio=8.03, P
ISSN:0021-8820
1881-1469
DOI:10.1038/ja.2015.34