An interplay between transcription, processing, and degradation determines tRNA levels in yeast

tRNA biogenesis in yeast involves the synthesis of the initial transcript by RNA polymerase III followed by processing and controlled degradation in both the nucleus and the cytoplasm. A vast landscape of regulatory elements controlling tRNA stability in yeast has emerged from recent studies. Divers...

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Published in:Wiley interdisciplinary reviews. RNA 2013-11, Vol.4 (6), p.709-722
Main Authors: Wichtowska, Dominika, Turowski, Tomasz W., Boguta, Magdalena
Format: Article
Language:English
Subjects:
Biodegradation
Cell Nucleus - genetics
Cytoplasm
Cytoplasm - genetics
DNA-directed RNA polymerase
Evolution
Exosome Multienzyme Ribonuclease Complex - genetics
Intermediates
Mutation
Nuclei
Regulatory sequences
RNA polymerase
RNA Polymerase III - genetics
RNA processing
RNA Processing, Post-Transcriptional - genetics
RNA Stability - genetics
RNA, Transfer - biosynthesis
RNA, Transfer - genetics
Saccharomyces cerevisiae - genetics
Surveillance
Transcription
Transcription, Genetic
tRNA
Yeast
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Summary:tRNA biogenesis in yeast involves the synthesis of the initial transcript by RNA polymerase III followed by processing and controlled degradation in both the nucleus and the cytoplasm. A vast landscape of regulatory elements controlling tRNA stability in yeast has emerged from recent studies. Diverse pathways of tRNA maturation generate multiple stable and unstable intermediates. A significant impact on tRNA stability is exerted by a variety of nucleotide modifications. Pre‐tRNAs are targets of exosome‐dependent surveillance in the nucleus. Some tRNAs that are hypomodified or bear specific destabilizing mutations are directed to the rapid tRNA decay pathway leading to 5′→3′ exonucleolytic degradation by Rat1 and Xrn1. tRNA molecules are selectively marked for degradation by a double CCA at their 3′ ends. In addition, under different stress conditions, tRNA half‐molecules can be generated by independent endonucleolytic cleavage events. Recent studies reveal unexpected relationships between the subsequent steps of tRNA biosynthesis and the mechanisms controlling its quality and turnover. WIREs RNA 2013, 4:709–722. doi: 10.1002/wrna.1190 This article is categorized under: RNA Processing > tRNA Processing RNA Turnover and Surveillance > Regulation of RNA Stability
ISSN:1757-7004
1757-7012
DOI:10.1002/wrna.1190