In Vitro Anti-HIV Activity of Partially Purified Coumarin(s) Isolated from Fungal Endophyte, Alternaria Species of Calophyllum inophyllum

Five totally different endophytic fungal species were isolated from bark and leaf parts of Calophyllum inophyllum. Leaf part yielded Trichoderma harzianum and Alternaria species, whereas bark showed the presence of Fusarium species, Aspergillus species and unidentified fungi. Two solvents (hexane an...

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Bibliographic Details
Published in:Pharmacology & pharmacy 2015-07, Vol.6 (7), p.321-328
Main Authors: Govindappa, Melappa, Hemmanur, Kavya C., Nithin, S., Chinna Poojari, Chandrappa, Bhat, Gopalakrishna, Channabasava, K.
Format: Article
Language:English
Subjects:
Alternaria
Aspergillus
Calophyllum inophyllum
Fusarium
Human immunodeficiency virus
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Summary:Five totally different endophytic fungal species were isolated from bark and leaf parts of Calophyllum inophyllum. Leaf part yielded Trichoderma harzianum and Alternaria species, whereas bark showed the presence of Fusarium species, Aspergillus species and unidentified fungi. Two solvents (hexane and methanol) were used for endophytic fungal extraction, and the Alternaria species had shown the presence of coumarin, whereas Trichoderma harzianum in methanol extract and Fusarium species in hexane extract had shown the coumarin(s) in all the four methods tested. The total coumarin yield was more in microwave assistance method, the methanol Alternaria species (3.941 plus or minus 0.082) stood first, followed by hexane extract of Alternaria species (3.254 plus or minus 0.082), Fusarium species (2.532 plus or minus 0.082) and Trichoderma harzianum (2.294 plus or minus 0.082), the plant extract showed 4.149 + 0.053. However, in order to know possible anti-HIV, it is necessary to isolate active coumarin from the Alternaria species, and the mechanism of action will be studied in future studies.
ISSN:2157-9423
2157-9431
DOI:10.4236/pp.2015.67034