A trifunctional, triangular RNA–protein complex

•Three RNA segments were designed to produce a trifunctional triangular shaped RNP.•The protein-attached segments assemble to form the RNP via kissing loop interactions.•The trifunctionalization of the RNP was confirmed empirically. Multifunctional molecular complexes are valuable tools with a varie...

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Bibliographic Details
Published in:FEBS letters 2015-08, Vol.589 (18), p.2424-2428
Main Authors: Ohuchi, Shoji J., Sagawa, Fumihiko, Sakamoto, Taiichi, Inoue, Tan
Format: Article
Language:English
Subjects:
Base Sequence
kissing loop
Kissing loop interaction
Models, Molecular
Nucleic Acid Conformation
Protein Binding
Ribosomal Proteins - chemistry
Ribosomal Proteins - metabolism
RNA - chemistry
RNA - genetics
RNA - metabolism
RNA–protein complex
RNP
RNP nano-object
Substrate Specificity
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Summary:•Three RNA segments were designed to produce a trifunctional triangular shaped RNP.•The protein-attached segments assemble to form the RNP via kissing loop interactions.•The trifunctionalization of the RNP was confirmed empirically. Multifunctional molecular complexes are valuable tools with a variety of applications. We have developed an RNA–protein complex (RNP) containing three different proteins attached to the tips of a triangular RNA scaffold. We designed and constructed three RNA strands that specifically bind a ribosomal protein, L7Ae, and that autonomously form a single triangular RNP via RNA kissing loop (KL) interactions. This RNP-based approach can be used as an alternative tool to produce unique, multifunctional molecules with customized dimensions, functions, and targets.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2015.07.005