Enzyme sensitivity towards high pressure at low temperature

The sensitivity of several selected enzymes, e.g. Bacillus subtilis alpha-amylase (BSA), peroxidase (POD), pectinmethylestetase (PME), polyphenoloxidase (PPO) and lipoxygenase (LOX) towards the combined action of high pressure (1 up to 4000 bar) and low temperature (-22 degrees C up to 0 degrees C)...

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Bibliographic Details
Published in:Food biotechnology 1998-01, Vol.12 (3), p.263-277
Main Authors: Indrawati, A. (Catholic University of Leuven, Heverlee, Belgium.), Loey, A. van, Hendrickx, M
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ALFA AMILASA
ALPHA AMYLASE
BACILLUS SUBTILIS
CATECHOL OXIDASE
CATECHOL OXYDASE
CATECOL OXIDASA
COLD
CONGELACION
CONGELATION
ENZYMIC ACTIVITY
FREEZING
FRIO
FROID
INACTIVATION
LIPOXIGENASA
LIPOXYGENASE
PECTINESTERASAS
PECTINESTERASE
PEROXIDASAS
PEROXIDASES
PEROXYDASE
PRESION
PRESSION
PRESSURE
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Summary:The sensitivity of several selected enzymes, e.g. Bacillus subtilis alpha-amylase (BSA), peroxidase (POD), pectinmethylestetase (PME), polyphenoloxidase (PPO) and lipoxygenase (LOX) towards the combined action of high pressure (1 up to 4000 bar) and low temperature (-22 degrees C up to 0 degrees C) was investigated. The effect of a high pressure/low temperature (HP/LT)/ treatment on the selected enzymes was compared to the effect of a freezing process at atmospheric pressure. It was found that (i) no irreversible inactivation of POD, PPO and BSA occurred after the HP/LT treatments tested. (ii) PME was only slightly irreversibly inactivated in the selected pressure and temperature domain (92% up to 80% of activity retention) and (iii) LOX was irreversibly inactivated to a large extent (3 up to 55% of activity retention) after the selected HP/LT treatments whereas a classical freezing at -18 degrees C and atmospheric pressure caused only a 10% decrease in LOX activity. It could be observed that the enzymic activity after the treatment of both PME and LOX slightly decreased during storage in an ice bath
ISSN:0890-5436
1532-4249
DOI:10.1080/08905439809549956