Regulation of the expression of the Epstein–Barr virus early gene BFRF1

The switch from latency to lytic phase of Epstein–Barr virus (EBV) is coordinated by the expression of two viral transactivators known as ZEBRA and RTA. The BFRF1 gene has been shown to be transcribed during the early phases of EBV lytic cycle. Here, we characterized the BFRF1 promoter showing that...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2006-03, Vol.347 (1), p.109-116
Main Authors: Granato, Marisa, Farina, Antonella, Gonnella, Roberta, Santarelli, Roberta, Frati, Luigi, Faggioni, Alberto, Angeloni, Antonio
Format: Article
Language:English
Subjects:
Base Sequence
BFRF1 regulation
Cell Line
DNA, Viral - genetics
DNA-Binding Proteins - genetics
DNA-Binding Proteins - physiology
Epstein-Barr virus
Gene Expression Regulation, Viral
Genes, Viral
Herpesvirus 4, Human - genetics
Herpesvirus 4, Human - pathogenicity
Herpesvirus 4, Human - physiology
Humans
Immediate-Early Proteins - genetics
Immediate-Early Proteins - physiology
Lytic cycle
Membrane Proteins - genetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Promoter Regions, Genetic
Trans-Activators - genetics
Trans-Activators - physiology
Transfection
Viral Proteins - genetics
Viral Proteins - physiology
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Summary:The switch from latency to lytic phase of Epstein–Barr virus (EBV) is coordinated by the expression of two viral transactivators known as ZEBRA and RTA. The BFRF1 gene has been shown to be transcribed during the early phases of EBV lytic cycle. Here, we characterized the BFRF1 promoter showing that ZEBRA transfection stimulated BFRF1 expression, whereas RTA induced BFRF1 only after the transfection of an amount of plasmid largely in excess than that sufficient to stimulate the expression of other RTA-responsive genes. However, a co-operative effect between ZEBRA and RTA in the expression of BFRF1 is evident since the transfection of RTA can rescue the transactivating capacity of a mutant of the ZEBRA protein, known as Z(S186A), that has a substitution affecting the DNA binding region. Moreover, we identified one ZEBRA-responsive element (ZRE) and one RTA-responsive element (RRE) within the BFRF1 promoter region.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2005.11.046