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Volatile anaesthetic halothane causes DNA damage in A549 lung cells
The present study was performed to elucidate the extent of damage and the ability of lung epithelial cells to recover or to undergo apoptosis after in vitro treatment with the volatile anaesthetic halothane. The results obtained from the comet assay clearly show that halothane, applied at 3.0 mM con...
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| Published in: | Toxicology (Amsterdam) 2006-08, Vol.20 (5), p.585-593 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c382t-1c35fd5e10becca2e5fe587b0abe081b681cbbfbec88a6bf1ea8344e2906c5c33 |
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| cites | cdi_FETCH-LOGICAL-c382t-1c35fd5e10becca2e5fe587b0abe081b681cbbfbec88a6bf1ea8344e2906c5c33 |
| container_end_page | 593 |
| container_issue | 5 |
| container_start_page | 585 |
| container_title | Toxicology (Amsterdam) |
| container_volume | 20 |
| creator | Topouzova-Hristova, T. Daza, P. Garcia-Herdugo, G. Stephanova, E. |
| description | The present study was performed to elucidate the extent of damage and the ability of lung epithelial cells to recover or to undergo apoptosis after in vitro treatment with the volatile anaesthetic halothane.
The results obtained from the comet assay clearly show that halothane, applied at 3.0
mM concentration, causes DNA and cell damage. Cells exhibited nuclear fragmentation and budding early after treatment and these events gradually increased during the next few days. The presence of a large number of mini-comets after single cell gel electrophoresis was found to represent apoptotic bodies with fragmented DNA.
Our results demonstrate apoptosis-like changes after in vitro exposure of A549 cells to the volatile anaesthetic halothane. The majority of the affected cells did not recover and were directed to cell death. |
| doi_str_mv | 10.1016/j.tiv.2005.10.004 |
| format | article |
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The results obtained from the comet assay clearly show that halothane, applied at 3.0
mM concentration, causes DNA and cell damage. Cells exhibited nuclear fragmentation and budding early after treatment and these events gradually increased during the next few days. The presence of a large number of mini-comets after single cell gel electrophoresis was found to represent apoptotic bodies with fragmented DNA.
Our results demonstrate apoptosis-like changes after in vitro exposure of A549 cells to the volatile anaesthetic halothane. The majority of the affected cells did not recover and were directed to cell death.</description><identifier>ISSN: 0887-2333</identifier><identifier>ISSN: 0300-483X</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2005.10.004</identifier><identifier>PMID: 16314068</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Anesthetics, Inhalation - toxicity ; Cell Line, Tumor ; Cell morphology ; Cell Nucleus - drug effects ; Comet assay ; DNA Damage - drug effects ; DNA integrity ; Halothane ; Halothane - toxicity ; Humans ; Lung - drug effects ; Lung - ultrastructure ; Lung cells ; Micronuclei, Chromosome-Defective</subject><ispartof>Toxicology (Amsterdam), 2006-08, Vol.20 (5), p.585-593</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-1c35fd5e10becca2e5fe587b0abe081b681cbbfbec88a6bf1ea8344e2906c5c33</citedby><cites>FETCH-LOGICAL-c382t-1c35fd5e10becca2e5fe587b0abe081b681cbbfbec88a6bf1ea8344e2906c5c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16314068$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Topouzova-Hristova, T.</creatorcontrib><creatorcontrib>Daza, P.</creatorcontrib><creatorcontrib>Garcia-Herdugo, G.</creatorcontrib><creatorcontrib>Stephanova, E.</creatorcontrib><title>Volatile anaesthetic halothane causes DNA damage in A549 lung cells</title><title>Toxicology (Amsterdam)</title><addtitle>Toxicol In Vitro</addtitle><description>The present study was performed to elucidate the extent of damage and the ability of lung epithelial cells to recover or to undergo apoptosis after in vitro treatment with the volatile anaesthetic halothane.
The results obtained from the comet assay clearly show that halothane, applied at 3.0
mM concentration, causes DNA and cell damage. Cells exhibited nuclear fragmentation and budding early after treatment and these events gradually increased during the next few days. The presence of a large number of mini-comets after single cell gel electrophoresis was found to represent apoptotic bodies with fragmented DNA.
Our results demonstrate apoptosis-like changes after in vitro exposure of A549 cells to the volatile anaesthetic halothane. The majority of the affected cells did not recover and were directed to cell death.</description><subject>Anesthetics, Inhalation - toxicity</subject><subject>Cell Line, Tumor</subject><subject>Cell morphology</subject><subject>Cell Nucleus - drug effects</subject><subject>Comet assay</subject><subject>DNA Damage - drug effects</subject><subject>DNA integrity</subject><subject>Halothane</subject><subject>Halothane - toxicity</subject><subject>Humans</subject><subject>Lung - drug effects</subject><subject>Lung - ultrastructure</subject><subject>Lung cells</subject><subject>Micronuclei, Chromosome-Defective</subject><issn>0887-2333</issn><issn>0300-483X</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EoqXwAWyQV-wSPHEerlhV5SlVsAG2lu1MWld5FDupxN_jqJXYsbJsn7m6cwi5BhYDg_xuG_d2HyeMZeEeM5aekCmIYh5xKIpTMmVCFFHCOZ-QC--3LIAiYedkAjmHlOViSpZfXa16WyNVrULfb7C3hm5U3fUb1SI1avDo6cPbgpaqUWuktqWLLJ3TemjX1GBd-0tyVqna49XxnJHPp8eP5Uu0en9-XS5WkeEi6SMwPKvKDIFpNEYlmFWYiUIzpZEJ0LkAo3UVPoVQua4AleBpismc5SYznM_I7SF357rvIZSVjfVjg1C0G7yEAkSacBFAOIDGdd47rOTO2Ua5HwlMjubkVgZzcjQ3PgVzYebmGD7oBsu_iaOqANwfAAwr7i066Y3F1mBpHZpelp39J_4X9Ip-Yg</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Topouzova-Hristova, T.</creator><creator>Daza, P.</creator><creator>Garcia-Herdugo, G.</creator><creator>Stephanova, E.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20060801</creationdate><title>Volatile anaesthetic halothane causes DNA damage in A549 lung cells</title><author>Topouzova-Hristova, T. ; Daza, P. ; Garcia-Herdugo, G. ; Stephanova, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-1c35fd5e10becca2e5fe587b0abe081b681cbbfbec88a6bf1ea8344e2906c5c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Anesthetics, Inhalation - toxicity</topic><topic>Cell Line, Tumor</topic><topic>Cell morphology</topic><topic>Cell Nucleus - drug effects</topic><topic>Comet assay</topic><topic>DNA Damage - drug effects</topic><topic>DNA integrity</topic><topic>Halothane</topic><topic>Halothane - toxicity</topic><topic>Humans</topic><topic>Lung - drug effects</topic><topic>Lung - ultrastructure</topic><topic>Lung cells</topic><topic>Micronuclei, Chromosome-Defective</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Topouzova-Hristova, T.</creatorcontrib><creatorcontrib>Daza, P.</creatorcontrib><creatorcontrib>Garcia-Herdugo, G.</creatorcontrib><creatorcontrib>Stephanova, E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Topouzova-Hristova, T.</au><au>Daza, P.</au><au>Garcia-Herdugo, G.</au><au>Stephanova, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Volatile anaesthetic halothane causes DNA damage in A549 lung cells</atitle><jtitle>Toxicology (Amsterdam)</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>20</volume><issue>5</issue><spage>585</spage><epage>593</epage><pages>585-593</pages><issn>0887-2333</issn><issn>0300-483X</issn><eissn>1879-3177</eissn><abstract>The present study was performed to elucidate the extent of damage and the ability of lung epithelial cells to recover or to undergo apoptosis after in vitro treatment with the volatile anaesthetic halothane.
The results obtained from the comet assay clearly show that halothane, applied at 3.0
mM concentration, causes DNA and cell damage. Cells exhibited nuclear fragmentation and budding early after treatment and these events gradually increased during the next few days. The presence of a large number of mini-comets after single cell gel electrophoresis was found to represent apoptotic bodies with fragmented DNA.
Our results demonstrate apoptosis-like changes after in vitro exposure of A549 cells to the volatile anaesthetic halothane. The majority of the affected cells did not recover and were directed to cell death.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16314068</pmid><doi>10.1016/j.tiv.2005.10.004</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0887-2333 |
| ispartof | Toxicology (Amsterdam), 2006-08, Vol.20 (5), p.585-593 |
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| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Anesthetics, Inhalation - toxicity Cell Line, Tumor Cell morphology Cell Nucleus - drug effects Comet assay DNA Damage - drug effects DNA integrity Halothane Halothane - toxicity Humans Lung - drug effects Lung - ultrastructure Lung cells Micronuclei, Chromosome-Defective |
| title | Volatile anaesthetic halothane causes DNA damage in A549 lung cells |
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