Alzheimer-specific epitope of AT100 in transfected cell lines with tau: toward an efficient cell model of tau abnormal phosphorylation

• Published in: Neuroscience letters 1998-10, Vol.255 (1), p.13-16
• Main Authors: Mailliot, Christel, Bussière, Thierry, Caillet-Boudin, Marie-Laure, Delacourte, André, Buée, Luc
• Format: Article
• Language: English
• Subjects: Alzheimer Disease - immunology; Alzheimer's disease; Animals; Antibodies, Monoclonal - immunology; Biological and medical sciences; Cell Line, Transformed; COS cells; COS Cells - drug effects; COS Cells - metabolism; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; DNA, Complementary - genetics; Electrophoresis, Polyacrylamide Gel; Epitopes - immunology; Epitopes - metabolism; Medical sciences; Neurology; Okadaic Acid - pharmacology; Phosphorylation; SY5Y neuroblastoma cells; tau protein; tau Proteins - genetics; tau Proteins - metabolism; Transfection; Transfection - genetics; Tumor Cells, Cultured - drug effects
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/S0304-3940(98)00693-4

Intraneuronal aggregation of specific hyperphosphorylated tau isoforms in subsets of neurons may explain many neurodegenerative processes. Only some antibodies including AP422 and AT100 are specific to the abnormal phosphorylation of tau proteins in these processes. AT100-immunoreactivity was never observed in cell models with the exception of Sf9 cells. In the present study, we developed a way to induce AT100-immunoreactivity in different cell types including COS and SY5Y cells after tau cDNA transfection and treatment by okadaic acid. This represents a useful model to study abnormal tau phosphorylation in situ.