Radiation damage to DNA-protein complexes

We review here the advances in understanding the effects of ionizing radiations on DNA, proteins and their complexes, resulting from the collaboration of the authors' teams. It concerns the preponderant indirect effect of low LET ionizing radiations, thus the attack of the macromolecules in aqu...

Full description

Saved in:
Bibliographic Details
Published in:Journal of physics. Conference series 2011-01, Vol.261 (1), p.012010-10
Main Authors: Spotheim-Maurizot, M, Davídková, M
Format: Article
Language:English
Subjects:
Amino acids
Aqueous solutions
Deoxyribonucleic acid
Electrophoresis
Fluorescence
Hydroxyl radicals
Ionizing radiation
Lesions
Macromolecules
Mass spectrometry
Mathematical models
Molecular dynamics
Molecular structure
Nucleotides
Physics
Proteins
Radiation damage
Radiolysis
Structural stability
Three dimensional
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We review here the advances in understanding the effects of ionizing radiations on DNA, proteins and their complexes, resulting from the collaboration of the authors' teams. It concerns the preponderant indirect effect of low LET ionizing radiations, thus the attack of the macromolecules in aqueous solution by the most aggressive product of water radiolysis, the hydroxyl radical. A model of simulation of the reaction of these radicals with the macromolecules (called RADACK) was developed and was used for calculating the probabilities of damage of each constituent of DNA or proteins (nucleotide or amino-acid). The calculations allowed to draw conclusions from electrophoresis, mutagenesis, spectroscopic (fluorescence, circular dichroism) and mass spectrometry experiments. Thus we have shown that the extent and location of the lesions are strongly dependent on the 3D structure of the macromolecules, which in turns is modulated by their sequence and by the binding of some ligands. Molecular dynamics simulation completed our studies in showing the consequences of each lesion on the stability and structure of the proteins and their complexes with DNA.
ISSN:1742-6596
1742-6588
1742-6596
DOI:10.1088/1742-6596/261/1/012010