Digital Microfluidic Approach for Efficient Electroporation with High Productivity: Transgene Expression of Microalgae without Cell Wall Removal

A unique digital microfluidic electroporation (EP) system successfully demonstrates higher transgene expression than that of conventional techniques, in addition to reliable productivity and feasible integrated processes. By systematic investigations into the effects of the droplet EP conditions for...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2015-07, Vol.87 (13), p.6592-6599
Main Authors: Im, Do Jin, Jeong, Su-Nam, Yoo, Byeong Sun, Kim, Bolam, Kim, Dong-Pyo, Jeong, Won-Joong, Kang, In Seok
Format: Article
Language:English
Subjects:
Analytical chemistry
Biotechnology
Cell culture
Cell Wall
Controllability
Digital
Droplets
Electroporation
Gene expression
Microalgae - genetics
Microfluidics
Microfluidics - methods
Productivity
Transgenes
Walls
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A unique digital microfluidic electroporation (EP) system successfully demonstrates higher transgene expression than that of conventional techniques, in addition to reliable productivity and feasible integrated processes. By systematic investigations into the effects of the droplet EP conditions for a wild-type microalgae, 1 order of magnitude higher transgene expression is accomplished without cell wall removal over the conventional bulk EP system. In addition, the newly proposed droplet EP method by a droplet contact charging phenomena shows a great potential for the integration of EP processes and on-chip cell culture providing easy controllability of each process. Finally, the implications of the accomplishments and future directions for development of the proposed technology are discussed.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.5b00725