Oligonucleotide microarray for subtyping of influenza A viruses

Influenza is one of the most widespread respiratory viral diseases, infecting humans, horses, pigs, poultry and some other animal populations. Influenza A viruses (IAV) are classified into subtypes on the basis of the surface hemagglutinin (H1 to H16) and neuraminidase (N1 to N9) glycoproteins. The...

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Bibliographic Details
Published in:Journal of physics. Conference series 2012-01, Vol.345 (1), p.1-7
Main Authors: Klotchenko, S A, Vasin, A V, Sandybaev, N T, Plotnikova, M A, Chervyakova, O V, Smirnova, E A, Kushnareva, E V, Strochkov, V M, Taylakova, E T, Egorov, Koshemetov, J K, Kiselev, O I, Sansyzbay, A R
Format: Article
Language:English
Subjects:
Fluorescence
Genes
Glycoproteins
Human
Influenza
Oligonucleotides
Physics
Poultry
Segments
Viral diseases
Viruses
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Summary:Influenza is one of the most widespread respiratory viral diseases, infecting humans, horses, pigs, poultry and some other animal populations. Influenza A viruses (IAV) are classified into subtypes on the basis of the surface hemagglutinin (H1 to H16) and neuraminidase (N1 to N9) glycoproteins. The correct determination of IAV subtype is necessary for clinical and epidemiological studies. In this article we propose an oligonucleotide microarray for subtyping of IAV using universal one-step multisegment RT-PCR fluorescent labeling of viral gene segments. It showed to be an advanced approach for fast detection and identification of IAV.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/345/1/012041