Influence of Cu–Ti thin film surface properties on antimicrobial activity and viability of living cells

• Published in: Materials Science & Engineering C 2015-11, Vol.56, p.48-56
• Main Authors: Wojcieszak, Damian, Kaczmarek, Danuta, Antosiak, Aleksandra, Mazur, Michal, Rybak, Zbigniew, Rusak, Agnieszka, Osekowska, Malgorzata, Poniedzialek, Agata, Gamian, Andrzej, Szponar, Bogumila
• Format: Article
• Language: English
• Subjects: ACTIVITY; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Antimicrobial; ANTISEPTICS; Bacteria; Bacteria - drug effects; Cell viability; CHEMICAL PROPERTIES; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; COATINGS; Copper; Copper - chemistry; Cu–Ti; Cytotoxic; Electron Probe Microanalysis - methods; Magnetron sputtering; Microscopy, Atomic Force - methods; Microscopy, Electron, Scanning - methods; Photoelectron Spectroscopy - methods; Surface chemistry; Surface Properties; SURFACES; Thin film; THIN FILMS; Titanium; Titanium - chemistry; Wettability; WETTING; X RAYS; X-Ray Diffraction - methods
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2015.06.013

The paper describes properties of thin-film coatings based on copper and titanium. Thin films were prepared by co-sputtering of Cu and Ti targets in argon plasma. Deposited coatings consist of 90at.% of Cu and 10at.% of Ti. Characterization of the film was made on the basis of investigations of microstructure and physicochemical properties of the surface. Methods such as scanning electron microscopy, x-ray microanalysis, x-ray diffraction, x-ray photoelectron spectroscopy, atomic force microscopy, optical profilometry and wettability measurements were used to assess the properties of deposited thin films. An impact of Cu–Ti coating on the growth of selected bacteria and viability of the living cells (line L929, NCTC clone 929) was described in relation to the structure, surface state and wettability of the film. It was found that as-deposited films were amorphous. However, in such surroundings the nanocrystalline grains of 10–15nm and 25–35nm size were present. High surface active area with a roughness of 8.9nm, had an effect on receiving relatively high water contact angle value (74.1°). Such wettability may promote cell adhesion and result in an increase of the probability of copper ion transfer from the film surface into the cell. Thin films revealed bactericidal and fungicidal effects even in short term-contact. High activity of prepared films was directly related to high amount (ca. 51 %) of copper ions at 1+ state as x-ray photoelectron spectroscopy results have shown. Bactericidal and fungicidal effects of time contact with surface of Cu–Ti thin films. [Display omitted] •Antimicrobial activity and cytotoxic effect (viability of L929 cell line) of metallic Cu–Ti films•Thin films were prepared by co-sputtering of Cu and Ti.•As-deposited Cu–Ti films were amorphous and homogenous.•Bactericidal and fungicidal effects even in short term-contact were observed.