Local delivery of COMP-angiopoietin 1 accelerates new bone formation in rat calvarial defects

Recombinant COMP‐Ang1, a chimera of angiopoietin‐1 (Ang1) and a short coiled‐coil domain of cartilage oligomeric matrix protein (COMP), is under consideration as a therapeutic agent in bone reconstruction. However, the potential of COMP‐Ang1 to regenerate impaired bone and induce new bone formation...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2015-09, Vol.103 (9), p.2942-2951
Main Authors: Lim, Shin-Saeng, Kook, Sung-Ho, Bhattarai, Govinda, Cho, Eui-Sic, Seo, Young-Kwon, Lee, Jeong-Chae
Format: Article
Language:English
Subjects:
Angiopoietin-1 - administration & dosage
Animals
Biocompatible Materials
Biomedical materials
bone regeneration
Bone Regeneration - drug effects
Bones
calvarial defect
Cartilage Oligomeric Matrix Protein - administration & dosage
Collagen
COMP-Ang1
Defects
Drug Delivery Systems
Equipment Failure Analysis
Formations
gene expression
Male
Markers
Neovascularization, Physiologic - drug effects
Neovascularization, Physiologic - genetics
Osteogenesis - drug effects
Osteogenesis - genetics
Rats
Rats, Sprague-Dawley
Recombinant
Recombinant Fusion Proteins - administration & dosage
RNA, Messenger - genetics
RNA, Messenger - metabolism
Scaffolds
Skull - drug effects
Skull - injuries
Skull - physiopathology
Surgery
Surgical Sponges
X-Ray Microtomography
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Summary:Recombinant COMP‐Ang1, a chimera of angiopoietin‐1 (Ang1) and a short coiled‐coil domain of cartilage oligomeric matrix protein (COMP), is under consideration as a therapeutic agent in bone reconstruction. However, the potential of COMP‐Ang1 to regenerate impaired bone and induce new bone formation has not been completely explored. In this study, male Sprague–Dawley rats underwent calvarial defect surgery and divided into two groups: scaffold treatment alone (control group) and COMP‐Ang1‐impregnated scaffold (COMP‐Ang1 group). According to live micro‐CT and histological analyses, the COMP‐Ang1 group showed greater new bone formation and maturation than did the control both four and eight weeks after surgery. The values of bone volume, bone mineral density, and bone surface were also higher in the COMP‐Ang1 group than in the control at the same weeks after surgery. In addition, the delivery of COMP‐Ang1 facilitated significantly the expression of osteoblast‐specific markers such as runt‐related transcription factor 2 (p 
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.35439