Covalent immobilization of nisin on multi-walled carbon nanotubes: superior antimicrobial and anti-biofilm properties

Despite unique and useful properties of multi-walled carbon nanotubes (MWNTs) such as high strength and a low synthesis cost, their weak antimicrobial property hampers their use as an antimicrobial material. Herein, we demonstrate that the immobilization of nisin, a natural and inexpensive antimicro...

Full description

Saved in:
Bibliographic Details
Published in:Nanoscale 2011-04, Vol.3 (4), p.1874-188
Main Authors: Qi, Xiaobao, Poernomo, Gunawan, Wang, Kean, Chen, Yuan, Chan-Park, Mary B, Xu, Rong, Chang, Matthew Wook
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemical synthesis
Anti-Bacterial Agents - pharmacology
Antiinfectives and antibacterials
Bacillus subtilis
Biofilms - drug effects
Biofilms - growth & development
Covalence
Deposits
Economics
Escherichia coli
Immobilization
Materials Testing
Multi wall carbon nanotubes
Nanotubes, Carbon - chemistry
Nanotubes, Carbon - ultrastructure
Nisin
Nisin - chemistry
Nisin - pharmacology
Pseudomonas aeruginosa
Staphylococcus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Despite unique and useful properties of multi-walled carbon nanotubes (MWNTs) such as high strength and a low synthesis cost, their weak antimicrobial property hampers their use as an antimicrobial material. Herein, we demonstrate that the immobilization of nisin, a natural and inexpensive antimicrobial peptide, with poly(ethylene glycol) (PEG 1000 ) as a linker significantly enhanced the antimicrobial and anti-biofilm properties of MWNTs. The MWNT-nisin composite showed up to 7-fold higher antimicrobial property than pristine MWNTs against Escherichia coli , Pseudomonas aeruginosa , Staphylococcus aureus and Bacillus subtilis . Moreover, the MWNT-nisin composite had a dramatically improved capability to prevent biofilm formation both on a deposited film and in suspension. In particular, the MWNT-nisin deposit film exhibited a 100-fold higher anti-biofilm property than the MWNT deposit film. Further, it has been shown that PEG and nisin are covalently attached to MWNTs with excellent stability against leaching. We envision that our novel MWNT-nisin composite can serve as an effective and economical antimicrobial material. Immobilization of nisin, an antimicrobial peptide, with PEG 1000 as a linker significantly enhances the antimicrobial and anti-biofilm properties of MWNTs.
ISSN:2040-3364
2040-3372
DOI:10.1039/c1nr10024f