Weak Cytokinins N,N'-bis-(1-naphthyl)urea and N,N'-bis-(2-naphthyl)urea may Enhance Rooting in Apple and Mung Bean

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2005-11, Vol.83 (2), p.179-186
• Main Authors: Ada, R, Angela, C, Enrico, R, Cristina, B, Camillo, B
• Format: Article
• Language: English
• Subjects: adventitious roots; apples; Biological and medical sciences; Biotechnology; culture media; cytokinins; dose response; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; in vitro culture; in vitro regeneration; Malus; Malus domestica; methodology; Methods. Procedures. Technologies; micropropagation; Miscellaneous; mung beans; Phaseolus aureus; Plant cells and fungal cells; plant cuttings; rooting; shoots; urea; Vigna radiata var. radiata
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-005-4931-2

The present research investigates the possibility that 2 weak urea-type cytokinins, the N,N'-bis-(1-naphthyl)urea and the N,N'-bis-(2-naphthyl)urea, enhance adventitious root formation. The rooting activity was assessed using the stem slice test, the mung bean rooting test and the rooting of apple microcuttings. The two compounds influenced the adventitious rooting process differently as regards the bioassay used. In the stem slice test, in the presence of exogenous auxin, both compounds enhanced the rooted slice percentage. In mung bean shoots, the N,N'-bis-(1-naphthyl)urea enhanced the root formation at the lowest concentration used (0.01 micromolar) while the N,N'-bis-(2-naphthyl)urea enhanced rooting at higher concentrations. In the rooting test of apple microcuttings the N,N'-bis-(1-naphthyl)urea and the N,N'-bis-(2-naphthyl)urea slightly enhanced only the mean root number per microcutting.