Micropropagation of Licorice (Glycyrrhiza glabra L.) through Shoot Tip and Nodal Cultures

Apical and axillary buds of Glycyrrhiza glabra commonly known as licorice, a plant of repute in the Indian system of medicine, were used for induction of adventitious shoots. For induction of multiple shoots, Murashige and Skoog's (MS) medium with N⁶-benzyladenine (BA, 0.88-8.87 μM) was used. R...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 1998-10, Vol.34 (4), p.331-334
Main Authors: Thengane, S. R., Kulkarni, D. K., Krishnamurthy, K. V.
Format: Article
Language:English
Subjects:
Adventitious shoots
Biological and medical sciences
Biotechnology
Charcoal
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Glycyrrhiza glabra
In vitro propagation: entire plant regeneration from tissues and cell cultures
Indian culture
Licorice
Methods. Procedures. Technologies
Micropropagation
Plant cells and fungal cells
Plant propagation
Plant roots
Plants
Salts
Sprouting
Subcultures
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Summary:Apical and axillary buds of Glycyrrhiza glabra commonly known as licorice, a plant of repute in the Indian system of medicine, were used for induction of adventitious shoots. For induction of multiple shoots, Murashige and Skoog's (MS) medium with N⁶-benzyladenine (BA, 0.88-8.87 μM) was used. Reduction in major salts of MS medium enhanced the multiplication ratio up to 1:10. Plants transferred to the greenhouse showed 90% survival. The present work describes a stepwise protocol for production of Glycyrrhiza glabra plants on simple minimal media, where very high multiplication rates with healthy root systems were obtained. Roots being the organ of commercial importance, the protocol has tremendous potential.
ISSN:1054-5476
1475-2689
DOI:10.1007/BF02822743