Role of astrocytic S100β in behavioral hypersensitivity in rodent models of neuropathic pain

• Published in: Neuroscience 2006, Vol.140 (3), p.1003-1010
• Main Authors: Tanga, F.Y., Raghavendra, V., Nutile-McMenemy, N., Marks, A., DeLeo, J.A.
• Format: Article
• Language: English
• Subjects: astrocytes; Biological and medical sciences; chronic pain; Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction; Fundamental and applied biological sciences. Psychology; glia; glial fibrillary acidic protein; mechanical allodynia; Medical sciences; Nervous system (semeiology, syndromes); Neurology; Vertebrates: nervous system and sense organs
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2006.02.070

S100β is a calcium-binding peptide produced mainly by astrocytes that exerts paracrine and autocrine effects on neurons and glia. We have previously shown that S100β is markedly elevated at the mRNA level in the spinal cord following peripheral inflammation, intraplantar administration of complete Freund’s adjuvant in the rat. The purpose of the present study was to further investigate the role of astrocytic S100β in mediating behavioral hypersensitivity in rodent models of persistent pain. First, we assessed the lumbar spinal cord expression of S100β at the mRNA and protein level using real-time RT-PCR, Western blot and immunohistochemistry analysis following L5 spinal nerve transection in rats, a rodent model of neuropathic pain. Second, we assessed behavioral hypersensitivity (mechanical allodynia) in wild type and genetically modified mice lacking or overexpressing S100β following L5 spinal nerve transection. Third, we assessed the expression level of S100β protein in the CD1 wild type mice after nerve injury. We report that lumbar spinal S100β mRNA steadily increased from days 4–28 after nerve injury. S100β protein in the lumbar spinal cord was significantly increased in both rats and mice at day 14 following nerve injury as compared with sham control groups. S100β genetically deficient mice displayed significantly increased tactile thresholds (reduced response to non-noxious stimuli) after nerve injury as compared with the wild type group. S100β overexpressing mice displayed significantly decreased tactile threshold responses (enhanced response to non-noxious stimuli). Together, these results from both series of experiments using a peripheral nerve injury model in two different species implicate the involvement of glial-derived S100β in the pathophysiology of neuropathic pain.